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Drosophila

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Cover of 'Drosophila'

Table of Contents

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    Book Overview
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    Chapter 1 Using FlyBase, a Database of Drosophila Genes and Genomes.
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    Chapter 2 The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression.
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    Chapter 3 The Q-System: A Versatile Expression System for Drosophila.
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    Chapter 4 Analysis of MicroRNA Function in Drosophila.
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    Chapter 5 Methods for High-Throughput RNAi Screening in Drosophila Cells.
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    Chapter 6 A Guide to Genome-Wide In Vivo RNAi Applications in Drosophila.
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    Chapter 7 Creating Heritable Mutations in Drosophila with CRISPR-Cas9.
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    Chapter 8 Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFP.
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    Chapter 9 deGradFP: A System to Knockdown GFP-Tagged Proteins.
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    Chapter 10 Drosophila
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    Chapter 11 Cultivation and Live Imaging of Drosophila Imaginal Discs.
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    Chapter 12 Cultivation and Live Imaging of Drosophila Ovaries.
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    Chapter 13 Segmentation and Quantitative Analysis of Epithelial Tissues.
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    Chapter 14 Laser Ablation to Probe the Epithelial Mechanics in Drosophila.
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    Chapter 15 Rapid Ovary Mass-Isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In Situ Hybridization.
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    Chapter 16 Drosophila
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    Chapter 17 Protocols to Study Growth and Metabolism in Drosophila.
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    Chapter 18 Protocols to Study Aging in Drosophila.
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    Chapter 19 Protocols to Study Behavior in Drosophila.
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    Chapter 20 Electrophysiological Recordings from Lobula Plate Tangential Cells in Drosophila.
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    Chapter 21 Methods to Establish Drosophila Cell Lines.
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    Chapter 22 Erratum to: The GAL4 System: A Versatile System for the Manipulation and Analysis of Gene Expression
Attention for Chapter 19: Protocols to Study Behavior in Drosophila.
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Chapter title
Protocols to Study Behavior in Drosophila.
Chapter number 19
Book title
Drosophila
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6371-3_19
Pubmed ID
Book ISBNs
978-1-4939-6369-0, 978-1-4939-6371-3
Authors

Wendi S. Neckameyer, Parag Bhatt

Editors

Christian Dahmann

Abstract

Drosophila melanogaster is an incredibly versatile organism capable of both innate and higher-order behaviors. These behaviors offer not only a way to assay whether or not the animal is physiologically compromised (e.g., feeding, locomotion), but also serve to assess changes in centrally mediated functions. Here we describe several high throughput, reproducible, yet inexpensive and facile behavioral assays for both larval and adult Drosophila. The larval assays all employ an agar substrate in a petri dish; the adult assays are grouped into "vial-based" and "arena-based" paradigms. While these protocols are largely designed to assess individual animals, they are sufficiently rapid that ample numbers can be tested to determine behavioral significance. Importantly, this also allows for one to control for reproductive status, age, and sex, since these factors all have a significant impact on adult behaviors. In general, it is best to designate a dedicated area for any assay, so that lighting conditions are consistent, and all animals should be tested at roughly the same time each day to minimize circadian fluctuations. Temperature and humidity should also be maintained at a constant level to minimize variability in the assays.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Canada 1 8%
Unknown 12 92%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 4 31%
Student > Ph. D. Student 3 23%
Researcher 2 15%
Unspecified 1 8%
Student > Master 1 8%
Other 2 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 46%
Biochemistry, Genetics and Molecular Biology 2 15%
Unspecified 1 8%
Nursing and Health Professions 1 8%
Sports and Recreations 1 8%
Other 2 15%