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Germline Stem Cells

Overview of attention for book
Cover of 'Germline Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of the C. elegans Germline Stem Cell Pool.
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    Chapter 2 Methods for Studying the Germline of the Human Parasite Schistosoma mansoni.
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    Chapter 3 Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells.
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    Chapter 4 Live Imaging of the Drosophila Testis Stem Cell Niche.
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    Chapter 5 RNA Isolation from Early Drosophila Larval Ovaries.
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    Chapter 6 Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
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    Chapter 7 Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells.
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    Chapter 8 Identification of Germ-Line Stem Cells in Zebrafish.
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    Chapter 9 Primordial Germ Cell Isolation from Xenopus laevis Embryos.
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    Chapter 10 Single-Cell Lineage Analysis of Oogenesis in Mice.
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    Chapter 11 Visualization and Lineage Tracing of Pax7(+) Spermatogonial Stem Cells in the Mouse.
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    Chapter 12 Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells.
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    Chapter 13 Mouse Fetal Germ Cell Isolation and Culture Techniques.
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    Chapter 14 Rattus norvegicus Spermatogenesis Colony-Forming Assays.
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    Chapter 15 Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies.
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    Chapter 16 Efficient Induction and Isolation of Human Primordial Germ Cell-Like Cells from Competent Human Pluripotent Stem Cells.
Attention for Chapter 6: Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
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Chapter title
Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
Chapter number 6
Book title
Germline Stem Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-4017-2_6
Pubmed ID
27734349
Book ISBNs
978-1-4939-4015-8, 978-1-4939-4017-2
Authors

Nevine A. Shalaby, Michael Buszczak

Editors

Michael Buszczak

Abstract

The Drosophila ovary represents a key in vivo model used to study germline stem cell (GSC) maintenance and stem cell daughter differentiation because these cells and their somatic cell neighbors can be identified at single-cell resolution within their native environment. Here we describe a fluorescent-based technique for the acquisition of 4D datasets of the Drosophila ovariole for periods that can exceed 12 consecutive hours. Live-cell imaging facilitates the investigation of molecular and cellular dynamics that were not previously possible using still images.

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Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 20%
Student > Master 2 20%
Other 1 10%
Student > Bachelor 1 10%
Unknown 4 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 30%
Agricultural and Biological Sciences 2 20%
Medicine and Dentistry 1 10%
Unknown 4 40%

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