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Germline Stem Cells

Overview of attention for book
Cover of 'Germline Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of the C. elegans Germline Stem Cell Pool.
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    Chapter 2 Methods for Studying the Germline of the Human Parasite Schistosoma mansoni.
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    Chapter 3 Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells.
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    Chapter 4 Live Imaging of the Drosophila Testis Stem Cell Niche.
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    Chapter 5 RNA Isolation from Early Drosophila Larval Ovaries.
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    Chapter 6 Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
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    Chapter 7 Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells.
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    Chapter 8 Identification of Germ-Line Stem Cells in Zebrafish.
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    Chapter 9 Primordial Germ Cell Isolation from Xenopus laevis Embryos.
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    Chapter 10 Single-Cell Lineage Analysis of Oogenesis in Mice.
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    Chapter 11 Visualization and Lineage Tracing of Pax7(+) Spermatogonial Stem Cells in the Mouse.
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    Chapter 12 Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells.
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    Chapter 13 Mouse Fetal Germ Cell Isolation and Culture Techniques.
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    Chapter 14 Rattus norvegicus Spermatogenesis Colony-Forming Assays.
  16. Altmetric Badge
    Chapter 15 Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies.
  17. Altmetric Badge
    Chapter 16 Efficient Induction and Isolation of Human Primordial Germ Cell-Like Cells from Competent Human Pluripotent Stem Cells.
Attention for Chapter 15: Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies.
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Chapter title
Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies.
Chapter number 15
Book title
Germline Stem Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-4017-2_15
Pubmed ID
27734358
Book ISBNs
978-1-4939-4015-8, 978-1-4939-4017-2
Authors

Takamasa Hirano, Hidetoshi Hasuwa, Haruhiko Siomi

Editors

Michael Buszczak

Abstract

The mouse testis has served as a popular model system to study a wide range of biological processes, including germ cell development, meiosis, epigenetic changes of chromatin, transposon silencing, and small RNA-mediated epigenetic modifications. PIWI-interacting RNAs (piRNAs) are a class of small RNAs that are almost exclusively expressed in animal gonads. They repress transposons by forming effector complexes with PIWI proteins to maintain genome integrity of the germline. Here we describe detailed procedures of how to produce monoclonal antibodies against a mouse nuclear PIWI protein, MIWI2, which functions in de novo DNA methylation of target transposon loci. We then describe how to use the antibodies to isolate associated complexes and to detect MIWI2 immunohistochemically.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 33%
Student > Master 2 22%
Student > Ph. D. Student 1 11%
Student > Bachelor 1 11%
Professor > Associate Professor 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 44%
Agricultural and Biological Sciences 3 33%
Unknown 2 22%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 January 2018.
All research outputs
#18,475,157
of 22,893,031 outputs
Outputs from Methods in molecular biology
#7,926
of 13,134 outputs
Outputs of similar age
#310,493
of 420,414 outputs
Outputs of similar age from Methods in molecular biology
#691
of 1,074 outputs
Altmetric has tracked 22,893,031 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,134 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 420,414 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 15th percentile – i.e., 15% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 21st percentile – i.e., 21% of its contemporaries scored the same or lower than it.

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