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Mendeley readers
| Chapter title |
High-Throughput and High-Sensitivity Mass Spectrometry-Based N-Glycomics of Mammalian Cells.
|
|---|---|
| Chapter number | 14 |
| Book title |
High-Throughput Glycomics and Glycoproteomics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6493-2_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6491-8, 978-1-4939-6493-2
|
| Authors |
Stephanie Holst, Gabi W. van Pelt, Wilma E. Mesker, Rob A. Tollenaar, Ana I. Belo, Irma van Die, Yoann Rombouts, Manfred Wuhrer |
| Editors |
Gordan Lauc, Manfred Wuhrer |
| Abstract |
The current protocols for glycomic analysis of cells often require a large quantity of material (5-20 million cells). In order to analyze the N-glycosylation from small amounts of cells (≤1 million) as obtained from, for example, primary cell lines or cell sorting, and in a higher throughput approach, we set up a robust 96-well format PVDF-membrane based N-glycan release protocol followed by linkage-specific sialic acid stabilization, cleanup, and MALDI-TOF-MS analysis. We further evaluated the influence of PNGase F incubation time on the N-glycan profile. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 25% |
| Lecturer | 1 | 25% |
| Other | 1 | 25% |
| Student > Doctoral Student | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Biochemistry, Genetics and Molecular Biology | 1 | 25% |
| Veterinary Science and Veterinary Medicine | 1 | 25% |