Title |
Comparative analysis of microbiome measurement platforms using latent variable structural equation modeling
|
---|---|
Published in |
BMC Bioinformatics, March 2013
|
DOI | 10.1186/1471-2105-14-79 |
Pubmed ID | |
Authors |
Xiao Wu, Kathryn Berkow, Daniel N Frank, Ellen Li, Ajay S Gulati, Wei Zhu |
Abstract |
Culture-independent phylogenetic analysis of 16S ribosomal RNA (rRNA) gene sequences has emerged as an incisive method of profiling bacteria present in a specimen. Currently, multiple techniques are available to enumerate the abundance of bacterial taxa in specimens, including the Sanger sequencing, the 'next generation' pyrosequencing, microarrays, quantitative PCR, and the rapidly emerging, third generation sequencing, and fourth generation sequencing methods. An efficient statistical tool is in urgent need for the followings tasks: (1) to compare the agreement between these measurement platforms, (2) to select the most reliable platform(s), and (3) to combine different platforms of complementary strengths, for a unified analysis. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 57% |
Germany | 1 | 14% |
France | 1 | 14% |
Unknown | 1 | 14% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 5 | 71% |
Members of the public | 2 | 29% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | 6% |
United Kingdom | 1 | 1% |
Japan | 1 | 1% |
Pakistan | 1 | 1% |
Unknown | 63 | 90% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 19 | 27% |
Student > Ph. D. Student | 18 | 26% |
Professor | 9 | 13% |
Student > Master | 5 | 7% |
Other | 4 | 6% |
Other | 11 | 16% |
Unknown | 4 | 6% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 26 | 37% |
Medicine and Dentistry | 11 | 16% |
Computer Science | 4 | 6% |
Business, Management and Accounting | 4 | 6% |
Biochemistry, Genetics and Molecular Biology | 3 | 4% |
Other | 16 | 23% |
Unknown | 6 | 9% |