↓ Skip to main content

Loss of Mgat5a-mediated N -glycosylation stimulates regeneration in zebrafish

Overview of attention for article published in Cell Regeneration, January 2016
Altmetric Badge

About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (56th percentile)

Mentioned by

twitter
4 tweeters

Citations

dimensions_citation
4 Dimensions

Readers on

mendeley
13 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Loss of Mgat5a-mediated N -glycosylation stimulates regeneration in zebrafish
Published in
Cell Regeneration, January 2016
DOI 10.1186/s13619-016-0031-5
Pubmed ID
Authors

Wuhong Pei, Sunny C. Huang, Lisha Xu, Kade Pettie, María Laura Ceci, Mario Sánchez, Miguel L. Allende, Shawn M. Burgess

Abstract

We are using genetics to identify genes specifically involved in hearing regeneration. In a large-scale genetic screening, we identified mgat5a, a gene in the N-glycosylation biosynthesis pathway whose activity negatively impacts hair cell regeneration. We used a combination of mutant analysis in zebrafish and a hair cell regeneration assay to phenotype the loss of Mgat5a activity in zebrafish. We used pharmacological inhibition of N-glycosylation by swansonine. We also used over-expression analysis by mRNA injections to demonstrate how changes in N-glycosylation can alter cell signaling. We found that mgat5a was expressed in multiple tissues during zebrafish embryo development, particularly enriched in neural tissues including the brain, retina, and lateral line neuromasts. An mgat5a insertional mutation and a CRISPR/Cas9-generated truncation mutation both caused an enhancement of hair cell regeneration which could be phenocopied by pharmacological inhibition with swansonine. In addition to hair cell regeneration, inhibition of the N-glycosylation pathway also enhanced the regeneration of lateral line axon and caudal fins. Further analysis showed that N-glycosylation altered the responsiveness of TGF-beta signaling. The findings from this study provide experimental evidence for the involvement of N-glycosylation in tissue regeneration and cell signaling.

Twitter Demographics

The data shown below were collected from the profiles of 4 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 23%
Student > Doctoral Student 2 15%
Student > Bachelor 2 15%
Professor 1 8%
Unspecified 1 8%
Other 3 23%
Unknown 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 46%
Agricultural and Biological Sciences 2 15%
Pharmacology, Toxicology and Pharmaceutical Science 1 8%
Unspecified 1 8%
Chemical Engineering 1 8%
Other 1 8%
Unknown 1 8%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 October 2016.
All research outputs
#3,725,395
of 8,549,553 outputs
Outputs from Cell Regeneration
#22
of 35 outputs
Outputs of similar age
#105,604
of 251,624 outputs
Outputs of similar age from Cell Regeneration
#1
of 1 outputs
Altmetric has tracked 8,549,553 research outputs across all sources so far. This one has received more attention than most of these and is in the 54th percentile.
So far Altmetric has tracked 35 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 10.9. This one scored the same or higher as 13 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 251,624 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.
We're also able to compare this research output to 1 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them