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Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption

Overview of attention for article published in BMC Research Notes, March 2013
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  • Above-average Attention Score compared to outputs of the same age and source (51st percentile)

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Title
Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption
Published in
BMC Research Notes, March 2013
DOI 10.1186/1756-0500-6-85
Pubmed ID
Authors

Sam Manna, Ashley Harman, Jessica Accari, Christian Barth

Abstract

The cloning of gene sequences forms the basis for many molecular biological studies. One important step in the cloning process is the isolation of bacterial transformants carrying vector DNA. This involves a vector-encoded selectable marker gene, which in most cases, confers resistance to an antibiotic. However, there are a number of circumstances in which a different selectable marker is required or may be preferable. Such situations can include restrictions to host strain choice, two phase cloning experiments and mutagenesis experiments, issues that result in additional unnecessary cloning steps, in which the DNA needs to be subcloned into a vector with a suitable selectable marker.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Portugal 1 1%
Unknown 81 99%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 26 32%
Student > Master 13 16%
Researcher 4 5%
Student > Ph. D. Student 4 5%
Student > Postgraduate 3 4%
Other 5 6%
Unknown 27 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 27 33%
Agricultural and Biological Sciences 13 16%
Immunology and Microbiology 4 5%
Unspecified 2 2%
Medicine and Dentistry 2 2%
Other 7 9%
Unknown 27 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2014.
All research outputs
#14,164,012
of 22,699,621 outputs
Outputs from BMC Research Notes
#1,947
of 4,255 outputs
Outputs of similar age
#111,630
of 194,888 outputs
Outputs of similar age from BMC Research Notes
#23
of 52 outputs
Altmetric has tracked 22,699,621 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,255 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one has gotten more attention than average, scoring higher than 50% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,888 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 52 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 51% of its contemporaries.