Title |
Altering the selection capabilities of common cloning vectors via restriction enzyme mediated gene disruption
|
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Published in |
BMC Research Notes, March 2013
|
DOI | 10.1186/1756-0500-6-85 |
Pubmed ID | |
Authors |
Sam Manna, Ashley Harman, Jessica Accari, Christian Barth |
Abstract |
The cloning of gene sequences forms the basis for many molecular biological studies. One important step in the cloning process is the isolation of bacterial transformants carrying vector DNA. This involves a vector-encoded selectable marker gene, which in most cases, confers resistance to an antibiotic. However, there are a number of circumstances in which a different selectable marker is required or may be preferable. Such situations can include restrictions to host strain choice, two phase cloning experiments and mutagenesis experiments, issues that result in additional unnecessary cloning steps, in which the DNA needs to be subcloned into a vector with a suitable selectable marker. |
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