| Chapter title |
Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy.
|
|---|---|
| Chapter number | 3 |
| Book title |
RNA Imaging
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3148-4_3 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3147-7, 978-1-4939-3148-4
|
| Authors |
Marie-France Penet, Zhihang Chen, Noriko Mori, Balaji Krishnamachary, Zaver M. Bhujwalla Ph.D., Zaver M. Bhujwalla |
| Editors |
Zdravka Medarova |
| Abstract |
Small interfering RNA (siRNA) is routinely used as a biological tool to silence specific genes, and is under active investigation in cancer treatment strategies. Noninvasive magnetic resonance spectroscopy (MRS) provides the ability to assess the functional effects of siRNA-mediated gene silencing in cultured cancer cells, and following nanoparticle-based delivery in tumors in vivo. Here we describe the use of siRNA to downregulate choline kinase, a critical enzyme in choline phospholipid metabolism of cancer cells and tumors, and the use of (1)H MRS of cells and (1)H magnetic resonance spectroscopic imaging (MRSI) of tumors to assess the efficacy of the downregulation. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 1 | 10% |
| Student > Ph. D. Student | 1 | 10% |
| Professor > Associate Professor | 1 | 10% |
| Student > Bachelor | 1 | 10% |
| Researcher | 1 | 10% |
| Other | 0 | 0% |
| Unknown | 5 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 1 | 10% |
| Immunology and Microbiology | 1 | 10% |
| Chemistry | 1 | 10% |
| Neuroscience | 1 | 10% |
| Unknown | 6 | 60% |