| Chapter title |
siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
|
|---|---|
| Chapter number | 9 |
| Book title |
RNA Imaging
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3148-4_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3147-7, 978-1-4939-3148-4
|
| Authors |
Seung Koo Lee, Ching-Hsuan Tung |
| Editors |
Zdravka Medarova |
| Abstract |
Small interfering RNA (siRNA)-mediated gene silencing has shown prominent therapeutic effects in treating various diseases. However, adequate delivery and persistent gene silencing remain challenging. A nanoparticle-based delivery system which assembled by layering siRNAs between protease degradable polypeptides to show ultra-long gene silencing effect in vivo is developed. Gold nanoparticle is used as a scaffold for its unique properties including uniform size, biocompatibility, ready synthesis, and easy functionalization. A simple layer-by-layer fabrication approach, based on the electrostatic interaction between positively and negatively charged polymers, is applied to package the therapeutic siRNAs. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 71% |
| Researcher | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 29% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Materials Science | 1 | 14% |
| Neuroscience | 1 | 14% |
| Unknown | 2 | 29% |