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RNA Imaging

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Cover of 'RNA Imaging'

Table of Contents

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    Book Overview
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    Chapter 1 Imaging Functional Nucleic Acid Delivery to Skin.
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    Chapter 2 In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets.
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    Chapter 3 Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy.
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    Chapter 4 Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
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    Chapter 5 RNA Imaging
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    Chapter 6 Indium-Labeling of siRNA for Small Animal SPECT Imaging.
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    Chapter 7 Imaging of Electrotransferred siRNA.
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    Chapter 8 Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents.
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    Chapter 9 siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
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    Chapter 10 Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
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    Chapter 11 Molecular Beacon-Based MicroRNA Imaging During Neurogenesis.
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    Chapter 12 Hypoxia-Responsive Copolymer for siRNA Delivery.
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    Chapter 13 Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging.
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    Chapter 14 Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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    Chapter 15 Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.
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    Chapter 16 MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery.
Attention for Chapter 14: Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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Chapter title
Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
Chapter number 14
Book title
RNA Imaging
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3148-4_14
Pubmed ID
Book ISBNs
978-1-4939-3147-7, 978-1-4939-3148-4
Authors

Valencia, Karmele, Lecanda, Fernando, Karmele Valencia, Fernando Lecanda Ph.D., Fernando Lecanda

Editors

Zdravka Medarova

Abstract

Microvesicles and exosomes are released to the extracellular milieu and are detectable in body fluids. They act as unique vehicles for cargo transfer to other cells/tissues. They contain a set of membrane receptors, intracellular proteins and nucleic acids. Here, we describe basic techniques for their isolation and characterization from cell culture media or body fluids. We also describe critical techniques for characterizing their cargo (miRNAs) and their protein content. Finally, we present labeling methods for their use in in vitro procedures and for their in vivo delivery and subsequent analysis of their cargo transfer to multiple cell types.

Mendeley readers

The data shown below were compiled from readership statistics for 36 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 3%
Unknown 35 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 36%
Student > Doctoral Student 6 17%
Researcher 5 14%
Student > Master 3 8%
Professor > Associate Professor 3 8%
Other 3 8%
Unknown 3 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 31%
Medicine and Dentistry 9 25%
Agricultural and Biological Sciences 7 19%
Neuroscience 2 6%
Veterinary Science and Veterinary Medicine 1 3%
Other 2 6%
Unknown 4 11%