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RNA Imaging

Overview of attention for book
Cover of 'RNA Imaging'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Imaging Functional Nucleic Acid Delivery to Skin.
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    Chapter 2 In Vivo Magnetic Resonance Imaging of Small Interfering RNA Nanodelivery to Pancreatic Islets.
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    Chapter 3 Magnetic Resonance Spectroscopy of siRNA-Based Cancer Therapy.
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    Chapter 4 Targeted Delivery with Imaging Assessment of siRNA Expressing Nanocassettes into Cancer.
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    Chapter 5 RNA Imaging
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    Chapter 6 Indium-Labeling of siRNA for Small Animal SPECT Imaging.
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    Chapter 7 Imaging of Electrotransferred siRNA.
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    Chapter 8 Whole-Body Scanning PCR, a Tool for the Visualization of the In Vivo Biodistribution Pattern of Endogenous and Exogenous Oligonucleotides in Rodents.
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    Chapter 9 siRNA Nanoparticles for Ultra-Long Gene Silencing In Vivo.
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    Chapter 10 Sensing miRNA: Signal Amplification by Cognate RISC for Intracellular Detection of miRNA in Live Cells.
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    Chapter 11 Molecular Beacon-Based MicroRNA Imaging During Neurogenesis.
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    Chapter 12 Hypoxia-Responsive Copolymer for siRNA Delivery.
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    Chapter 13 Controlling RNA Expression in Cancer Using Iron Oxide Nanoparticles Detectable by MRI and In Vivo Optical Imaging.
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    Chapter 14 Microvesicles: Isolation, Characterization for In Vitro and In Vivo Procedures.
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    Chapter 15 Positive Bioluminescence Imaging of MicroRNA Expression in Small Animal Models Using an Engineered Genetic-Switch Expression System, RILES.
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    Chapter 16 MicroRNA Imaging in Combination with Diagnostic Ultrasound and Bubble Liposomes for MicroRNA Delivery.
Attention for Chapter 1: Imaging Functional Nucleic Acid Delivery to Skin.
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Chapter title
Imaging Functional Nucleic Acid Delivery to Skin.
Chapter number 1
Book title
RNA Imaging
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3148-4_1
Pubmed ID
Book ISBNs
978-1-4939-3147-7, 978-1-4939-3148-4
Authors

Kaspar, Roger L, Hickerson, Robyn P, González-González, Emilio, Flores, Manuel A, Speaker, Tycho P, Rogers, Faye A, Milstone, Leonard M, Contag, Christopher H, Roger L. Kaspar, Robyn P. Hickerson, Emilio González-González, Manuel A. Flores, Tycho P. Speaker, Faye A. Rogers, Leonard M. Milstone, Christopher H. Contag

Editors

Zdravka Medarova

Abstract

Monogenic skin diseases arise from well-defined single gene mutations, and in some cases a single point mutation. As the target cells are superficial, these diseases are ideally suited for treatment by nucleic acid-based therapies as well as monitoring through a variety of noninvasive imaging technologies. Despite the accessibility of the skin, there remain formidable barriers for functional delivery of nucleic acids to the target cells within the dermis and epidermis. These barriers include the stratum corneum and the layered structure of the skin, as well as more locally, the cellular, endosomal and nuclear membranes. A wide range of technologies for traversing these barriers has been described and moderate success has been reported for several approaches. The lessons learned from these studies include the need for combinations of approaches to facilitate nucleic acid delivery across these skin barriers and then functional delivery across the cellular and nuclear membranes for expression (e.g., reporter genes, DNA oligonucleotides or shRNA) or into the cytoplasm for regulation (e.g., siRNA, miRNA, antisense oligos). The tools for topical delivery that have been evaluated include chemical, physical and electrical methods, and the development and testing of each of these approaches has been greatly enabled by imaging tools. These techniques allow delivery and real time monitoring of reporter genes, therapeutic nucleic acids and also triplex nucleic acids for gene editing. Optical imaging is comprised of a number of modalities based on properties of light-tissue interaction (e.g., scattering, autofluorescence, and reflectance), the interaction of light with specific molecules (e.g., absorbtion, fluorescence), or enzymatic reactions that produce light (bioluminescence). Optical imaging technologies operate over a range of scales from macroscopic to microscopic and if necessary, nanoscopic, and thus can be used to assess nucleic acid delivery to organs, regions, cells and even subcellular structures. Here we describe the animal models, reporter genes, imaging approaches and general strategies for delivery of nucleic acids to cells in the skin for local expression (e.g., plasmid DNA) or gene silencing (e.g., siRNA) with the intent of developing nucleic acid-based therapies to treat diseases of the skin.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 27%
Researcher 3 27%
Professor 1 9%
Student > Master 1 9%
Student > Ph. D. Student 1 9%
Other 0 0%
Unknown 2 18%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 36%
Medicine and Dentistry 2 18%
Pharmacology, Toxicology and Pharmaceutical Science 1 9%
Social Sciences 1 9%
Biochemistry, Genetics and Molecular Biology 1 9%
Other 1 9%
Unknown 1 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 November 2015.
All research outputs
#5,575,153
of 6,535,212 outputs
Outputs from Methods in molecular biology
#3,066
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Outputs of similar age
#167,321
of 210,012 outputs
Outputs of similar age from Methods in molecular biology
#232
of 354 outputs
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