Chapter title |
RNA Imaging
|
---|---|
Chapter number | 5 |
Book title |
RNA Imaging
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3148-4_5 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3147-7, 978-1-4939-3148-4
|
Authors |
Byrne, Frances L, McCarroll, Joshua A, Kavallaris, Maria, Frances L. Byrne, Joshua A. McCarroll, Maria Kavallaris, Byrne, Frances L., McCarroll, Joshua A. |
Editors |
Zdravka Medarova |
Abstract |
Cancer xenograft mouse models are useful for examining and understanding tumor growth and cancer progression in vivo. With the development of bioluminescent imaging, these parameters can now be monitored noninvasively with relative ease. Herein we describe imaging of luciferase-expressing cancer cells to quantitatively measure tumor burden in vivo and metastases ex vivo. Specifically, we detail the methodology to examine the effect of shRNA-mediated knockdown of a target gene on the growth and spread of neuroblastoma tumors in immune-deficient mice. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 40% |
Researcher | 3 | 15% |
Student > Bachelor | 1 | 5% |
Other | 1 | 5% |
Student > Master | 1 | 5% |
Other | 1 | 5% |
Unknown | 5 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 25% |
Medicine and Dentistry | 3 | 15% |
Agricultural and Biological Sciences | 2 | 10% |
Immunology and Microbiology | 2 | 10% |
Neuroscience | 1 | 5% |
Other | 0 | 0% |
Unknown | 7 | 35% |