Chapter title |
Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System.
|
---|---|
Chapter number | 8 |
Book title |
Long Non-Coding RNAs
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3378-5_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3376-1, 978-1-4939-3378-5
|
Authors |
Tan, John C, Bouriakov, Venera D, Feng, Liang, Richmond, Todd A, Burgess, Daniel, John C. Tan, Venera D. Bouriakov, Liang Feng, Todd A. Richmond, Daniel Burgess |
Editors |
Yi Feng, Lin Zhang |
Abstract |
Sequencing-based whole-transcriptome analysis (i.e., RNA-Seq) can be a powerful tool when used to measure gene expression, detect novel transcripts, characterize transcript isoforms, and identify sequence polymorphisms. However, this method can be inefficient when the goal is to study only one component of the transcriptome, such as long noncoding RNAs (lncRNAs), which constitute only a small fraction of transcripts in a total RNA sample. Here, we describe a target enrichment method where a total RNA sample is converted to a sequencing-ready cDNA library and hybridized to a complex pool of lncRNA-specific biotinylated long oligonucleotide capture probes prior to sequencing. The resulting sequence data are highly enriched for the targets of interest, dramatically increasing the efficiency of next-generation sequencing approaches for the analysis of lncRNAs. |
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