Chapter title |
Targeting Long Noncoding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics.
|
---|---|
Chapter number | 16 |
Book title |
Long Non-Coding RNAs
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3378-5_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3376-1, 978-1-4939-3378-5
|
Authors |
Zhou, Tianyuan, Kim, Youngsoo, MacLeod, A Robert, Tianyuan Zhou, Youngsoo Kim, A. Robert MacLeod |
Editors |
Yi Feng, Lin Zhang |
Abstract |
Recent annotation of the human transcriptome revealed that only 2 % of the genome encodes proteins while the majority of human genome is transcribed into noncoding RNAs. Although we are just beginning to understand the diverse roles long noncoding RNAs (lncRNAs) play in molecular and cellular processes, they have potentially important roles in human development and pathophysiology. However, targeting of RNA by traditional structure-based design of small molecule inhibitors has been difficult, due to a lack of understanding of the dynamic tertiary structures most RNA molecules adopt. Antisense oligonucleotides (ASOs) are capable of targeting specific genes or transcripts directly through Watson-Crick base pairing and thus can be designed based on sequence information alone. These agents have made possible specific targeting of "non-druggable targets" including RNA molecules. Here we describe how ASOs can be applied in preclinical studies to reduce levels of lncRNAs of interest. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 4 | 17% |
Researcher | 3 | 13% |
Other | 2 | 9% |
Professor > Associate Professor | 2 | 9% |
Student > Ph. D. Student | 2 | 9% |
Other | 4 | 17% |
Unknown | 6 | 26% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 8 | 35% |
Agricultural and Biological Sciences | 3 | 13% |
Neuroscience | 2 | 9% |
Chemistry | 2 | 9% |
Unknown | 8 | 35% |