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Long Non-Coding RNAs

Overview of attention for book
Cover of 'Long Non-Coding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins.
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    Chapter 2 Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions Between Long Noncoding RNAs and RNA-Binding Proteins.
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    Chapter 3 Characterization of Long Noncoding RNA-Associated Proteins by RNA-Immunoprecipitation.
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    Chapter 4 Isolation of Protein Complexes Associated with Long Noncoding RNAs.
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    Chapter 5 Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray.
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    Chapter 6 Long Noncoding RNA Expression Profiling Using Arraystar LncRNA Microarrays.
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    Chapter 7 Nuclear RNA Isolation and Sequencing.
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    Chapter 8 Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System.
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    Chapter 9 ChIP-Seq: Library Preparation and Sequencing.
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    Chapter 10 Stellaris(®) RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells.
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    Chapter 11 Simultaneous RNA-DNA FISH.
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    Chapter 12 Visualizing Long Noncoding RNAs on Chromatin.
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    Chapter 13 Non-isotopic Method for In Situ LncRNA Visualization and Quantitation.
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    Chapter 14 Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots.
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    Chapter 15 Assessment of In Vivo siRNA Delivery in Cancer Mouse Models.
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    Chapter 16 Targeting Long Noncoding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics.
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    Chapter 17 Characterization of Circular RNAs.
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    Chapter 18 Methods for Characterization of Alternative RNA Splicing.
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    Chapter 19 NONCODEv4: Annotation of Noncoding RNAs with Emphasis on Long Noncoding RNAs.
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    Chapter 20 Computational Analysis of LncRNA from cDNA Sequences.
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    Chapter 21 Analyzing MiRNA-LncRNA Interactions.
  23. Altmetric Badge
    Chapter 22 Long Noncoding RNAs: An Overview.
Attention for Chapter 12: Visualizing Long Noncoding RNAs on Chromatin.
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Chapter title
Visualizing Long Noncoding RNAs on Chromatin.
Chapter number 12
Book title
Long Non-Coding RNAs
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3378-5_12
Pubmed ID
26721489
Book ISBNs
978-1-4939-3376-1, 978-1-4939-3378-5
Authors

Hinten, Michael, Maclary, Emily, Gayen, Srimonta, Harris, Clair, Kalantry, Sundeep, Michael Hinten, Emily Maclary, Srimonta Gayen, Clair Harris, Sundeep Kalantry

Editors

Yi Feng, Lin Zhang

Abstract

Fluorescence in situ hybridization (FISH) enables the detection of specific nucleic acid sequences within single cells. For example, RNA FISH provides information on both the expression level and localization of RNA transcripts and, when combined with detection of associated proteins and chromatin modifications, can lend essential insights into long noncoding RNA (lncRNA) function. Epigenetic effects have been postulated for many lncRNAs, but shown for only a few. Advances in in situ techniques and microscopy, however, now allow for visualization of lncRNAs that are expressed at very low levels or are not very stable. FISH-based detections of RNA and DNA coupled with immunological staining of proteins/histone modifications offer the possibility to connect lncRNAs to epigenetic effects. Here, we describe an integrated set of protocols to detect, individually or in combination, specific RNAs, DNAs, proteins, and histone modifications in single cells at a high level of sensitivity using conventional fluorescence microscopy.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 38 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 38 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 18%
Student > Master 6 16%
Researcher 6 16%
Student > Doctoral Student 3 8%
Professor > Associate Professor 2 5%
Other 3 8%
Unknown 11 29%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 13 34%
Agricultural and Biological Sciences 8 21%
Neuroscience 4 11%
Immunology and Microbiology 1 3%
Chemistry 1 3%
Other 0 0%
Unknown 11 29%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 January 2016.
All research outputs
#18,433,196
of 22,836,570 outputs
Outputs from Methods in molecular biology
#7,921
of 13,126 outputs
Outputs of similar age
#284,426
of 393,564 outputs
Outputs of similar age from Methods in molecular biology
#846
of 1,470 outputs
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So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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