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Long Non-Coding RNAs

Overview of attention for book
Cover of 'Long Non-Coding RNAs'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins.
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    Chapter 2 Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions Between Long Noncoding RNAs and RNA-Binding Proteins.
  4. Altmetric Badge
    Chapter 3 Characterization of Long Noncoding RNA-Associated Proteins by RNA-Immunoprecipitation.
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    Chapter 4 Isolation of Protein Complexes Associated with Long Noncoding RNAs.
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    Chapter 5 Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray.
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    Chapter 6 Long Noncoding RNA Expression Profiling Using Arraystar LncRNA Microarrays.
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    Chapter 7 Nuclear RNA Isolation and Sequencing.
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    Chapter 8 Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System.
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    Chapter 9 ChIP-Seq: Library Preparation and Sequencing.
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    Chapter 10 Stellaris(®) RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells.
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    Chapter 11 Simultaneous RNA-DNA FISH.
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    Chapter 12 Visualizing Long Noncoding RNAs on Chromatin.
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    Chapter 13 Non-isotopic Method for In Situ LncRNA Visualization and Quantitation.
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    Chapter 14 Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots.
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    Chapter 15 Assessment of In Vivo siRNA Delivery in Cancer Mouse Models.
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    Chapter 16 Targeting Long Noncoding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics.
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    Chapter 17 Characterization of Circular RNAs.
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    Chapter 18 Methods for Characterization of Alternative RNA Splicing.
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    Chapter 19 NONCODEv4: Annotation of Noncoding RNAs with Emphasis on Long Noncoding RNAs.
  21. Altmetric Badge
    Chapter 20 Computational Analysis of LncRNA from cDNA Sequences.
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    Chapter 21 Analyzing MiRNA-LncRNA Interactions.
  23. Altmetric Badge
    Chapter 22 Long Noncoding RNAs: An Overview.
Attention for Chapter 18: Methods for Characterization of Alternative RNA Splicing.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (81st percentile)
  • High Attention Score compared to outputs of the same age and source (88th percentile)

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Citations

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142 Mendeley
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Chapter title
Methods for Characterization of Alternative RNA Splicing.
Chapter number 18
Book title
Long Non-Coding RNAs
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3378-5_18
Pubmed ID
Book ISBNs
978-1-4939-3376-1, 978-1-4939-3378-5
Authors

Harvey, Samuel E, Cheng, Chonghui, Samuel E. Harvey, Chonghui Cheng, Harvey, Samuel E.

Editors

Yi Feng, Lin Zhang

Abstract

Quantification of alternative splicing to detect the abundance of differentially spliced isoforms of a gene in total RNA can be accomplished via RT-PCR using both quantitative real-time and semi-quantitative PCR methods. These methods require careful PCR primer design to ensure specific detection of particular splice isoforms. We also describe analysis of alternative splicing using a splicing "minigene" in mammalian cell tissue culture to facilitate investigation of the regulation of alternative splicing of a particular exon of interest.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 142 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 142 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 32 23%
Student > Bachelor 25 18%
Researcher 14 10%
Student > Master 14 10%
Student > Doctoral Student 11 8%
Other 13 9%
Unknown 33 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 58 41%
Agricultural and Biological Sciences 22 15%
Medicine and Dentistry 5 4%
Neuroscience 4 3%
Immunology and Microbiology 4 3%
Other 12 8%
Unknown 37 26%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 August 2021.
All research outputs
#4,133,231
of 22,837,982 outputs
Outputs from Methods in molecular biology
#1,096
of 13,126 outputs
Outputs of similar age
#70,469
of 393,564 outputs
Outputs of similar age from Methods in molecular biology
#163
of 1,470 outputs
Altmetric has tracked 22,837,982 research outputs across all sources so far. Compared to these this one has done well and is in the 81st percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 13,126 research outputs from this source. They receive a mean Attention Score of 3.4. This one has done particularly well, scoring higher than 91% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,564 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 81% of its contemporaries.
We're also able to compare this research output to 1,470 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.