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Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

Overview of attention for article published in Biotechnology for Biofuels, October 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (75th percentile)
  • Good Attention Score compared to outputs of the same age and source (76th percentile)

Mentioned by

twitter
11 tweeters

Citations

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31 Dimensions

Readers on

mendeley
138 Mendeley
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Title
Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
Published in
Biotechnology for Biofuels, October 2016
DOI 10.1186/s13068-016-0638-3
Pubmed ID
Authors

Shilpa Nagaraju, Naomi Kathleen Davies, David Jeffrey Fraser Walker, Michael Köpke, Séan Dennis Simpson

Abstract

Impactful greenhouse gas emissions abatement can now be achieved through gas fermentation using acetogenic microbes for the production of low-carbon fuels and chemicals. However, compared to traditional hosts like Escherichia coli or yeast, only basic genetic tools exist for gas-fermenting acetogens. To advance the process, a robust genetic engineering platform for acetogens is essential. In this study, we report scarless genome editing of an industrially used model acetogen, Clostridium autoethanogenum, using the CRISPR/Cas9 system. Initial efforts to retrofit the CRISPR/Cas9 system for C. autoethanogenum resulted in poor efficiency likely due to uncontrolled expression of Cas9. To address this, we constructed and screened a small library of tetracycline-inducible promoters that can also be used to fine-tune gene expression. With a new inducible promoter, the efficiency of CRISPR/Cas9-mediated desired gene deletion in C. autoethanogenum was improved to over 50 %, making it a viable tool for engineering C. autoethanogenum. Addition of both an inducible promoter library and a scarless genome editing tool is an important expansion to the genetic tool box of industrial C. autoethanogenum strain.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 138 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 <1%
United States 1 <1%
New Zealand 1 <1%
Unknown 135 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 33 24%
Researcher 28 20%
Unspecified 20 14%
Student > Master 15 11%
Student > Bachelor 10 7%
Other 32 23%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 49 36%
Agricultural and Biological Sciences 45 33%
Unspecified 25 18%
Engineering 5 4%
Immunology and Microbiology 4 3%
Other 10 7%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 October 2017.
All research outputs
#1,954,685
of 11,918,812 outputs
Outputs from Biotechnology for Biofuels
#168
of 919 outputs
Outputs of similar age
#63,221
of 258,572 outputs
Outputs of similar age from Biotechnology for Biofuels
#10
of 43 outputs
Altmetric has tracked 11,918,812 research outputs across all sources so far. Compared to these this one has done well and is in the 83rd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 919 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done well, scoring higher than 81% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 258,572 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 75% of its contemporaries.
We're also able to compare this research output to 43 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 76% of its contemporaries.