| Chapter title |
Synthetic DNA
|
|---|---|
| Chapter number | 5 |
| Book title |
Synthetic DNA
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6343-0_5 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6341-6, 978-1-4939-6343-0
|
| Authors |
Currin, Andrew, Swainston, Neil, Day, Philip J, Kell, Douglas B, Andrew Currin, Neil Swainston, Philip J. Day, Douglas B. Kell |
| Editors |
Randall A. Hughes |
| Abstract |
Gene synthesis is a fundamental technology underpinning much research in the life sciences. In particular, synthetic biology and biotechnology utilize gene synthesis to assemble any desired DNA sequence, which can then be incorporated into novel parts and pathways. Here, we describe SpeedyGenes, a gene synthesis method that can assemble DNA sequences with greater fidelity (fewer errors) than existing methods, but that can also be used to encode extensive, statistically designed sequence variation at any position in the sequence to create diverse (but accurate) variant libraries. We summarize the integrated use of GeneGenie to design DNA and oligonucleotide sequences, followed by the procedure for assembling these accurately and efficiently using SpeedyGenes. |
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Mendeley readers
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