Chapter title |
Synthetic DNA
|
---|---|
Chapter number | 16 |
Book title |
Synthetic DNA
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6343-0_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6341-6, 978-1-4939-6343-0
|
Authors |
Jajesniak, Pawel, Wong, Tuck Seng, Pawel Jajesniak, Tuck Seng Wong |
Editors |
Randall A. Hughes |
Abstract |
QuickStep-Cloning is a novel molecular cloning technique that builds upon the concepts of asymmetric PCR and megaprimer-based amplification of whole plasmid. It was designed specifically to address the major drawbacks of previously reported cloning methods. The fully optimized protocol allows for a seamless integration of a long DNA fragment into any position within a plasmid of choice, in a time-efficient and cost-effective manner, without the need of a tedious DNA gel purification, a restriction digestion, and an enzymatic ligation. QuickStep-Cloning can be completed in less than 6 h, significantly faster than most of the existing cloning methods, while retaining high efficiency. |
X Demographics
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Demographic breakdown
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 28 | 90% |
Demographic breakdown
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Researcher | 6 | 19% |
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Student > Master | 3 | 10% |
Unspecified | 2 | 6% |
Other | 1 | 3% |
Unknown | 8 | 26% |
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Unspecified | 2 | 6% |
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Chemical Engineering | 1 | 3% |
Other | 3 | 10% |
Unknown | 9 | 29% |