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Synthetic DNA

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Cover of 'Synthetic DNA'

Table of Contents

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    Book Overview
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    Chapter 1 A Guide to Using STITCHER for Overlapping Assembly PCR Applications.
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    Chapter 2 Synthetic Gene Design Using Codon Optimization On-Line (COOL).
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    Chapter 3 Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering.
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    Chapter 4 Simple Cloning by Prolonged Overlap Extension-PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli.
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    Chapter 5 Synthetic DNA
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    Chapter 6 BASIC: A Simple and Accurate Modular DNA Assembly Method.
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    Chapter 7 Enzymatic Synthesis of Single-Stranded Clonal Pure Oligonucleotides.
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    Chapter 8 Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).
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    Chapter 9 PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly.
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    Chapter 10 The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis.
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    Chapter 11 Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.
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    Chapter 12 Generation of DNA Constructs Using the Golden GATEway Cloning Method.
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    Chapter 13 Synthetic DNA
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    Chapter 14 Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).
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    Chapter 15 Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions.
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    Chapter 16 Synthetic DNA
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    Chapter 17 Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA.
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    Chapter 18 Selection of Error-Less Synthetic Genes in Yeast.
Attention for Chapter 11: Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.
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Chapter title
Clonetegration Using OSIP Plasmids: One-Step DNA Assembly and Site-Specific Genomic Integration in Bacteria.
Chapter number 11
Book title
Synthetic DNA
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6343-0_11
Pubmed ID
Book ISBNs
978-1-4939-6341-6, 978-1-4939-6343-0
Authors

Lun Cui, Keith E. Shearwin, Cui, Lun, Shearwin, Keith E.

Editors

Randall A. Hughes

Abstract

Clonetegration is a method for site-specific insertion of DNA into prokaryotic chromosomes, based on bacteriophage integrases. The method combines DNA cloning/assembly and chromosomal integration into a single step, providing a simple and rapid strategy for inserting DNA sequences into bacterial chromosomes.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 2%
China 1 2%
Unknown 47 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 13 27%
Researcher 12 24%
Student > Master 6 12%
Student > Bachelor 3 6%
Student > Postgraduate 2 4%
Other 6 12%
Unknown 7 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 20 41%
Agricultural and Biological Sciences 9 18%
Chemical Engineering 6 12%
Immunology and Microbiology 2 4%
Unspecified 1 2%
Other 4 8%
Unknown 7 14%