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Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution

Overview of attention for article published in BMC Genomics, October 2016
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (74th percentile)
  • High Attention Score compared to outputs of the same age and source (83rd percentile)

Mentioned by

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11 tweeters

Citations

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45 Dimensions

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84 Mendeley
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Title
Sex-linked markers in the North American green frog (Rana clamitans) developed using DArTseq provide early insight into sex chromosome evolution
Published in
BMC Genomics, October 2016
DOI 10.1186/s12864-016-3209-x
Pubmed ID
Authors

Max R. Lambert, David K. Skelly, Tariq Ezaz

Abstract

The extent to which sex reversal is associated with transitions in sex determining systems (XX-XY, ZZ-ZW, etc.) or abnormal sexual differentiation is predominantly unexplored in amphibians. This is in large part because most amphibian taxa have homomorphic sex chromosomes, which has traditionally made it challenging to identify discordance between phenotypic and genetic sex in amphibians, despite all amphibians having a genetic component to sex determination. Recent advances in molecular techniques such as genome complexity reduction and high throughput sequencing present a valuable avenue for furthering our understanding of sex determination in amphibians and other taxa with homomorphic sex chromosomes like many fish and reptiles. We use DArTseq as a novel approach to identify sex-linked markers in the North American green frog (Rana clamitans melanota) using lab-reared tadpoles as well as wild-caught adults from seven ponds either in undeveloped, forested habitats or suburban ponds known to be subject to contamination by anthropogenic chemicals. The DArTseq methodology identified 13 sex-linked SNP loci and eight presence-absence loci associated with males, indicating an XX-XY system. Both alleles from a single locus show partial high sequence homology to Dmrt1, a gene linked to sex determination and differentiation throughout Metazoa. Two other loci have sequence similarities to regions of the chimpanzee and human X-chromosome as well as the chicken Z-chromosome. Several loci also show geographic variation in sex-linkage, possibly indicating sex chromosome recombination. While all loci are statistically sex-linked, they show varying degrees of female heterozygosity and male homozygosity, providing further evidence that some markers are on regions of the sex chromosomes undergoing higher rates of recombination and therefore further apart from the putative sex determining locus. The ease of the DArTseq platform provides a useful avenue for future research on sex reversal and sex chromosome evolution in vertebrates, particularly for non-model species with homomorphic or cryptic or nascent sex chromosomes.

Twitter Demographics

The data shown below were collected from the profiles of 11 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 84 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 1%
Netherlands 1 1%
United States 1 1%
Unknown 81 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 19 23%
Researcher 15 18%
Student > Master 11 13%
Student > Bachelor 7 8%
Student > Postgraduate 6 7%
Other 12 14%
Unknown 14 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 32 38%
Biochemistry, Genetics and Molecular Biology 25 30%
Environmental Science 8 10%
Veterinary Science and Veterinary Medicine 1 1%
Psychology 1 1%
Other 1 1%
Unknown 16 19%

Attention Score in Context

This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2017.
All research outputs
#3,776,733
of 19,194,973 outputs
Outputs from BMC Genomics
#1,716
of 9,727 outputs
Outputs of similar age
#77,986
of 305,578 outputs
Outputs of similar age from BMC Genomics
#149
of 877 outputs
Altmetric has tracked 19,194,973 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 9,727 research outputs from this source. They receive a mean Attention Score of 4.5. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,578 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 877 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.