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Viral infection modulation and neutralization by camelid nanobodies

Overview of attention for article published in Proceedings of the National Academy of Sciences of the United States of America, March 2013
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1 tweeter

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64 Mendeley
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Title
Viral infection modulation and neutralization by camelid nanobodies
Published in
Proceedings of the National Academy of Sciences of the United States of America, March 2013
DOI 10.1073/pnas.1301336110
Pubmed ID
Authors

A. Desmyter, C. Farenc, J. Mahony, S. Spinelli, C. Bebeacua, S. Blangy, D. Veesler, D. van Sinderen, C. Cambillau

Abstract

Lactococcal phages belong to a large family of Siphoviridae and infect Lactococcus lactis, a gram-positive bacterium used in commercial dairy fermentations. These phages are believed to recognize and bind specifically to pellicle polysaccharides covering the entire bacterium. The phage TP901-1 baseplate, located at the tip of the tail, harbors 18 trimeric receptor binding proteins (RBPs) promoting adhesion to a specific lactococcal strain. Phage TP901-1 adhesion does not require major conformational changes or Ca(2+), which contrasts other lactococcal phages. Here, we produced and characterized llama nanobodies raised against the purified baseplate and the Tal protein of phage TP901-1 as tools to dissect the molecular determinants of phage TP901-1 infection. Using a set of complementary techniques, surface plasmon resonance, EM, and X-ray crystallography in a hybrid approach, we identified binders to the three components of the baseplate, analyzed their affinity for their targets, and determined their epitopes as well as their functional impact on TP901-1 phage infectivity. We determined the X-ray structures of three nanobodies in complex with the RBP. Two of them bind to the saccharide binding site of the RBP and are able to fully neutralize TP901-1 phage infectivity, even after 15 passages. These results provide clear evidence for a practical use of nanobodies in circumventing lactococcal phages viral infection in dairy fermentation.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 64 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 64 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 17 27%
Student > Ph. D. Student 14 22%
Student > Bachelor 6 9%
Student > Master 4 6%
Other 3 5%
Other 11 17%
Unknown 9 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 19 30%
Agricultural and Biological Sciences 17 27%
Immunology and Microbiology 4 6%
Medicine and Dentistry 3 5%
Veterinary Science and Veterinary Medicine 3 5%
Other 7 11%
Unknown 11 17%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 August 2014.
All research outputs
#7,726,683
of 12,365,166 outputs
Outputs from Proceedings of the National Academy of Sciences of the United States of America
#68,321
of 77,340 outputs
Outputs of similar age
#78,104
of 144,819 outputs
Outputs of similar age from Proceedings of the National Academy of Sciences of the United States of America
#875
of 1,087 outputs
Altmetric has tracked 12,365,166 research outputs across all sources so far. This one is in the 23rd percentile – i.e., 23% of other outputs scored the same or lower than it.
So far Altmetric has tracked 77,340 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 22.0. This one is in the 6th percentile – i.e., 6% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 144,819 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,087 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.