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Monitoring sea lamprey pheromones and their degradation using rapid stream-side extraction coupled with UPLC-MS/MS

Overview of attention for article published in Journal of Separation Science (JSS) (formerly Journal of High Resolution Chromatography), April 2013
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Title
Monitoring sea lamprey pheromones and their degradation using rapid stream-side extraction coupled with UPLC-MS/MS
Published in
Journal of Separation Science (JSS) (formerly Journal of High Resolution Chromatography), April 2013
DOI 10.1002/jssc.201300110
Pubmed ID
Authors

Huiyong Wang, Nicholas Johnson, Jeffrey Bernardy, Terry Hubert, Weiming Li

Abstract

Pheromones guide adult sea lamprey (Petromyzon marinus) to suitable spawning streams and mates, and therefore, when quantified, can be used to assess population size and guide management. Here, we present an efficient sample preparation method where 100 mL of river water was spiked with deuterated pheromone as an internal standard and underwent rapid field-based SPE and elution in the field. The combination of field extraction with laboratory UPLC-MS/MS reduced the sample consumption from 1 to 0.1 L, decreased the sample process time from more than 1 h to 10 min, and increased the precision and accuracy. The sensitivity was improved more than one order of magnitude compared with the previous method. The influences of experimental conditions were assessed to optimize the separation and peak shapes. The analytical method has been validated by studies of stability, selectivity, precision, and linearity and by the determination of the limits of detection and quantification. The method was used to quantify pheromone concentration from five streams tributary to Lake Ontario and to estimate that the environmental half-life of 3kPZS is about 26 h.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 40%
Student > Master 2 20%
Other 2 20%
Student > Ph. D. Student 1 10%
Unknown 1 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 40%
Chemistry 2 20%
Environmental Science 1 10%
Unknown 3 30%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 March 2013.
All research outputs
#9,742,809
of 12,189,304 outputs
Outputs from Journal of Separation Science (JSS) (formerly Journal of High Resolution Chromatography)
#767
of 1,798 outputs
Outputs of similar age
#95,498
of 136,472 outputs
Outputs of similar age from Journal of Separation Science (JSS) (formerly Journal of High Resolution Chromatography)
#4
of 35 outputs
Altmetric has tracked 12,189,304 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,798 research outputs from this source. They receive a mean Attention Score of 1.7. This one is in the 22nd percentile – i.e., 22% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 136,472 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.