Mammary Gland Development
Springer New York
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Timeline
Mendeley readers
| Chapter title |
A 3D Culture Model to Study How Fluid Pressure and Flow Affect the Behavior of Aggregates of Epithelial Cells.
|
|---|---|
| Chapter number | 12 |
| Book title |
Mammary Gland Development
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6475-8_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6473-4, 978-1-4939-6475-8
|
| Authors |
Alexandra S. Piotrowski-Daspit, Allison K. Simi, Mei-Fong Pang, Joe Tien, Celeste M. Nelson |
| Editors |
Finian Martin, Torsten Stein, Jillian Howlin |
| Abstract |
Cells are surrounded by mechanical stimuli in their microenvironment. It is important to determine how cells respond to the mechanical information that surrounds them in order to understand both development and disease progression, as well as to be able to predict cell behavior in response to physical stimuli. Here we describe a protocol to determine the effects of interstitial fluid flow on the migratory behavior of an aggregate of epithelial cells in a three-dimensional (3D) culture model. This protocol includes detailed methods for the fabrication of a 3D cell culture chamber with hydrostatic pressure control, the culture of epithelial cells as an aggregate in a collagen gel, and the analysis of collective cell behavior in response to pressure-driven flow. |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 5% |
| Unknown | 18 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 26% |
| Researcher | 4 | 21% |
| Student > Doctoral Student | 2 | 11% |
| Other | 1 | 5% |
| Professor | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 3 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 21% |
| Engineering | 3 | 16% |
| Chemical Engineering | 2 | 11% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Unspecified | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 4 | 21% |