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The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens

Overview of attention for article published in SpringerPlus, March 2013
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7 Mendeley
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Title
The passage of cells can improve the detection rate of avian leukosis virus to facilitate the elimination of avian leukosis in chickens
Published in
SpringerPlus, March 2013
DOI 10.1186/2193-1801-2-138
Pubmed ID
Authors

Xiuzhen Wang, Bo Wang, Peipei Zhang, Hegang Cheng, Shuhong Sun

Abstract

Avian leukosis (AL) is one of the most harmful diseases to the poultry industry in China. The detection of the avian leukosis virus (ALV) p27 antigen plays a decisive role in the elimination of avian leukosis. To explore the influence of passaging cells on the detection rate of the ALV p27 antigen, 21 aseptic anticoagulated blood samples were collected from 21 chickens for which the cloacal swabs were positive for the p27 antigen to inoculate two sets of cell culture plates containing DF1 cells. The cells were cultured for 4 d, one set was passaged, and the other set was not. After the DF1 cells had been cultured for 9 d, the ALV p27 antigen in the supernatants of the two sets was detected by ELISA. The results showed that the p27 antigen-positive rate for the passaged cells was 71.43% (15/21), higher than that of the cells that were directly cultured, which was 42.86%. There was a strong correlation, as high as 0.928, with respect to the S/P value of the p27 antigen in the supernatant between the two sets. In conclusion, there was a strong correlation between the results for the passaged and unpassaged cells, and the passage of cells greatly improved the detection of the p27 antigen.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 14%
Unknown 6 86%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 2 29%
Researcher 1 14%
Student > Bachelor 1 14%
Lecturer > Senior Lecturer 1 14%
Student > Master 1 14%
Other 1 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 29%
Agricultural and Biological Sciences 2 29%
Earth and Planetary Sciences 1 14%
Engineering 1 14%
Unknown 1 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 March 2013.
All research outputs
#20,187,333
of 22,703,044 outputs
Outputs from SpringerPlus
#1,461
of 1,852 outputs
Outputs of similar age
#173,511
of 198,759 outputs
Outputs of similar age from SpringerPlus
#71
of 136 outputs
Altmetric has tracked 22,703,044 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,852 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 198,759 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 136 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.