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Cancer Gene Networks

Overview of attention for book
Cover of 'Cancer Gene Networks'

Table of Contents

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    Book Overview
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    Chapter 1 Introduction: Cancer Gene Networks.
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    Chapter 2 Emerging Methods in Chemoproteomics with Relevance to Drug Discovery.
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    Chapter 3 ANXA7-GTPase as Tumor Suppressor: Mechanisms and Therapeutic Opportunities.
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    Chapter 4 Experimental and Study Design Considerations for Uncovering Oncometabolites.
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    Chapter 5 Targeting Deubiquitinating Enzymes and Autophagy in Cancer.
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    Chapter 6 Quantitative Clinical Imaging Methods for Monitoring Intratumoral Evolution.
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    Chapter 7 Transcriptome and Proteome Analyses of TNFAIP8 Knockdown Cancer Cells Reveal New Insights into Molecular Determinants of Cell Survival and Tumor Progression.
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    Chapter 8 Network-Oriented Approaches to Anticancer Drug Response.
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    Chapter 9 CRISPR/Cas-Mediated Knockin in Human Pluripotent Stem Cells.
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    Chapter 10 Complete Transcriptome RNA-Seq.
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    Chapter 11 Computational Methods and Correlation of Exon-skipping Events with Splicing, Transcription, and Epigenetic Factors.
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    Chapter 12 Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma.
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    Chapter 13 microRNA Target Prediction.
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    Chapter 14 Evaluating the Delivery of Proteins to the Cytosol of Mammalian Cells.
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    Chapter 15 Validation of Biomarker Proteins Using Reverse Capture Protein Microarrays.
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    Chapter 16 Chemical Synthesis of Activity-Based Diubiquitin Probes.
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    Chapter 17 Profiling the Dual Enzymatic Activities of the Serine/Threonine Kinase IRE1α.
Attention for Chapter 9: CRISPR/Cas-Mediated Knockin in Human Pluripotent Stem Cells.
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Chapter title
CRISPR/Cas-Mediated Knockin in Human Pluripotent Stem Cells.
Chapter number 9
Book title
Cancer Gene Networks
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6539-7_9
Pubmed ID
Book ISBNs
978-1-4939-6537-3, 978-1-4939-6539-7
Authors

Nipun Verma, Zengrong Zhu, Danwei Huangfu

Editors

Usha Kasid, Robert Clarke

Abstract

Fluorescent reporter and epitope-tagged human pluripotent stem cells (hPSCs) greatly facilitate studies on the pluripotency and differentiation characteristics of these cells. Unfortunately traditional procedures to generate such lines are hampered by a low targeting efficiency that necessitates a lengthy process of selection followed by the removal of the selection cassette. Here we describe a procedure to generate fluorescent reporter and epitope tagged hPSCs in an efficient one-step process using the CRISPR/Cas technology. Although the method described uses our recently developed iCRISPR platform, the protocols can be adapted for general use with CRISPR/Cas or other engineered nucleases. The transfection procedures described could also be used for additional applications, such as overexpression or lineage tracing studies.

Mendeley readers

The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 33 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 18%
Student > Ph. D. Student 5 15%
Researcher 5 15%
Student > Master 4 12%
Professor > Associate Professor 3 9%
Other 4 12%
Unknown 6 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 33%
Agricultural and Biological Sciences 5 15%
Engineering 3 9%
Neuroscience 2 6%
Immunology and Microbiology 1 3%
Other 4 12%
Unknown 7 21%