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Chapter title |
Chemical Synthesis of Activity-Based Diubiquitin Probes.
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Chapter number | 16 |
Book title |
Cancer Gene Networks
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6539-7_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6537-3, 978-1-4939-6539-7
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Authors |
Guorui Li, Libo Yuan, Zhihao Zhuang |
Editors |
Usha Kasid, Robert Clarke |
Abstract |
Activity-based diubiquitin probes are highly useful in probing the deubiquitinase (DUB) activity and ubiquitin chain linkage specificity. Here we describe a detailed protocol to synthesize a new class of diubiquitin DUB probes. In this method, two ubiquitin moieties are connected through a linker resembling the native linkage in size and containing a Michael acceptor for trapping the DUB active-site cysteine. Detailed procedures for generating the linker molecule are also described. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 33% |
Student > Postgraduate | 1 | 33% |
Student > Master | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 100% |