↓ Skip to main content

Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region ofGeomyces destructans

Overview of attention for article published in Mycologia, January 2017
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age

Mentioned by

twitter
2 tweeters
facebook
1 Facebook page

Citations

dimensions_citation
101 Dimensions

Readers on

mendeley
95 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Bat white-nose syndrome: a real-time TaqMan polymerase chain reaction test targeting the intergenic spacer region ofGeomyces destructans
Published in
Mycologia, January 2017
DOI 10.3852/12-242
Pubmed ID
Authors

Laura K. Muller, Jeffrey M. Lorch, Daniel L. Lindner, Michael O’Connor, Andrea Gargas, David S. Blehert

Abstract

The fungus Geomyces destructans is the causative agent of white-nose syndrome (WNS), a disease that has killed millions of North American hibernating bats. We describe a real-time TaqMan PCR test that detects DNA from G. destructans by targeting a portion of the multicopy intergenic spacer region of the rRNA gene complex. The test is highly sensitive, consistently detecting as little as 3.3 fg genomic DNA from G. destructans. The real-time PCR test specifically amplified genomic DNA from G. destructans but did not amplify target sequence from 54 closely related fungal isolates (including 43 Geomyces spp. isolates) associated with bats. The test was qualified further by analyzing DNA extracted from 91 bat wing skin samples, and PCR results matched histopathology findings. These data indicate the real-time TaqMan PCR method described herein is a sensitive, specific and rapid test to detect DNA from G. destructans and provides a valuable tool for WNS diagnostics and research.

Twitter Demographics

The data shown below were collected from the profiles of 2 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 95 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 5 5%
Unknown 90 95%

Demographic breakdown

Readers by professional status Count As %
Student > Master 23 24%
Researcher 20 21%
Student > Ph. D. Student 15 16%
Student > Bachelor 8 8%
Other 8 8%
Other 16 17%
Unknown 5 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 51 54%
Veterinary Science and Veterinary Medicine 10 11%
Environmental Science 10 11%
Biochemistry, Genetics and Molecular Biology 6 6%
Immunology and Microbiology 3 3%
Other 6 6%
Unknown 9 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 May 2013.
All research outputs
#7,549,759
of 12,519,766 outputs
Outputs from Mycologia
#1,471
of 1,695 outputs
Outputs of similar age
#73,135
of 144,499 outputs
Outputs of similar age from Mycologia
#9
of 10 outputs
Altmetric has tracked 12,519,766 research outputs across all sources so far. This one is in the 37th percentile – i.e., 37% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,695 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 144,499 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 10 others from the same source and published within six weeks on either side of this one.