Selecting Optimal Peptides for Targeted Proteomic Experiments in Human Plasma Using In Vitro Synthesized Proteins as Analytical Standards.
Quantitative Proteomics by Mass Spectrometry
Methods in molecular biology, January 2016
James G. Bollinger, Andrew B. Stergachis, Richard S. Johnson, Jarrett D. Egertson, Michael J. MacCoss
In targeted proteomics, the development of robust methodologies is dependent upon the selection of a set of optimal peptides for each protein-of-interest. Unfortunately, predicting which peptides and respective product ion transitions provide the greatest signal-to-noise ratio in a particular assay matrix is complicated. Using in vitro synthesized proteins as analytical standards, we report here an empirically driven method for the selection of said peptides in a human plasma assay matrix.
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