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MicroRNA Profiling

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Cover of 'MicroRNA Profiling'

Table of Contents

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    Book Overview
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    Chapter 1 MiRNA Biogenesis and Regulation of Diseases: An Overview.
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    Chapter 2 Assessment of Basic Biological Functions Exerted by miRNAs.
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    Chapter 3 Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues.
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    Chapter 4 MicroRNA Profiling
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    Chapter 5 MicroRNA Profiling of Exosomes.
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    Chapter 6 MiRNA Profiling in Human Induced Pluripotent Stem Cells.
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    Chapter 7 MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells.
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    Chapter 8 TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery.
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    Chapter 9 Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.
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    Chapter 10 Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.
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    Chapter 11 Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.
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    Chapter 12 Circulating MicroRNAs in Cancer.
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    Chapter 13 Profiling Circulating miRNAs from the Plasma of Individuals with Metabolic Syndrome.
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    Chapter 14 Manipulating MiRNA Expression to Uncover Hidden Functions.
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    Chapter 15 Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation.
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    Chapter 16 MicroRNA Expression Profiling Using Agilent One-Color Microarray.
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    Chapter 17 A Multiplex Ligation Assay for miRNA Copy Number Profiling.
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    Chapter 18 Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.
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    Chapter 19 Visualization and Analysis of MiRNA-Targets Interactions Networks.
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    Chapter 20 Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.
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    Chapter 21 Customization of Artificial MicroRNA Design.
Attention for Chapter 6: MiRNA Profiling in Human Induced Pluripotent Stem Cells.
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Chapter title
MiRNA Profiling in Human Induced Pluripotent Stem Cells.
Chapter number 6
Book title
MicroRNA Profiling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6524-3_6
Pubmed ID
Book ISBNs
978-1-4939-6522-9, 978-1-4939-6524-3
Authors

Erica Hennessy

Editors

Sweta Rani

Abstract

Human iPS cells are capable of differentiation towards all three germ layer lineages. As well as being of use for the study of developmental biology, these cells also provide an excellent resource for disease modeling and cell replacement therapies. iPS cells derived from an individual with a disease type of interest can be differentiated towards the cell type afflicted in that particular disease. Such differentiated cell types can be used to test patient-specific drug responses in vitro, for generation of replacement cells for transplantation, or as a starting material for gene editing to correct disease-causing mutations/deletions. With such a vast array of potential applications, there is a great need to understand the exact mechanisms controlling the maintenance of pluripotency, and the distinct cues signaling these cells to differentiate towards lineages of interest. This chapter focuses on microRNA profiling using TaqMan Human MicroRNA Arrays to examine expression of 754 miRNAs in human iPS cells in the pluripotent state, and iPS derived definitive endoderm.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Librarian 1 25%
Other 1 25%
Student > Doctoral Student 1 25%
Unknown 1 25%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 25%
Agricultural and Biological Sciences 1 25%
Sports and Recreations 1 25%
Unknown 1 25%