Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.
Methods in molecular biology, January 2017
Sean Quinlan, Christine Henke, Gary P. Brennan, David C. Henshall, Eva M. Jimenez-Mateos
MicroRNAs are key posttranscriptional regulators of protein levels in cells. The brain is particularly enriched in microRNAs, and important roles have been demonstrated for these noncoding RNAs in various neurological disorders. To this end, visualization of microRNAs in specific cell types and subcellular compartments within tissue sections provides researchers with essential insights that support understanding of the cell and molecular mechanisms of microRNAs in brain diseases. In this chapter we describe an in situ hybridization protocol for the detection of microRNAs in mouse brain sections, which provides cellular resolution of the expression of microRNAs in the brain.
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