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Mendeley readers
Chapter title |
Manipulating MiRNA Expression to Uncover Hidden Functions.
|
---|---|
Chapter number | 14 |
Book title |
MicroRNA Profiling
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Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6524-3_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6522-9, 978-1-4939-6524-3
|
Authors |
Sinéad T. Aherne, Nga T. Lao |
Editors |
Sweta Rani |
Abstract |
There are a numerous target prediction algorithms that allow users to identify putative targets of their microRNAs (miRNAs) of interest. Although these tools are useful to gain insight into the potential role of miRNAs in regulating cellular processes, physical manipulation of the expression of the miRNA is the most superior way to truly determine the function of the miRNA in the system of interest. This chapter outlines methods to reveal miRNA function by modulating miRNA expression by transient transfection of miRNA mimics and inhibitors, and stable overexpression and reduction of miRNA expression using plasmid overexpression and sponge vectors. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 1 | 33% |
Other | 1 | 33% |
Unknown | 1 | 33% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 67% |
Unknown | 1 | 33% |