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MicroRNA Profiling

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Cover of 'MicroRNA Profiling'

Table of Contents

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    Book Overview
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    Chapter 1 MiRNA Biogenesis and Regulation of Diseases: An Overview.
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    Chapter 2 Assessment of Basic Biological Functions Exerted by miRNAs.
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    Chapter 3 Extraction of miRNAs from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues.
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    Chapter 4 MicroRNA Profiling
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    Chapter 5 MicroRNA Profiling of Exosomes.
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    Chapter 6 MiRNA Profiling in Human Induced Pluripotent Stem Cells.
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    Chapter 7 MiRNA Expression in Cystic Fibrosis Bronchial Epithelial Cells.
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    Chapter 8 TaqMan Low Density Array: MicroRNA Profiling for Biomarker and Oncosuppressor Discovery.
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    Chapter 9 Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.
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    Chapter 10 Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.
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    Chapter 11 Profiling the MicroRNA Payload of Exosomes Derived from Ex Vivo Primary Colorectal Fibroblasts.
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    Chapter 12 Circulating MicroRNAs in Cancer.
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    Chapter 13 Profiling Circulating miRNAs from the Plasma of Individuals with Metabolic Syndrome.
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    Chapter 14 Manipulating MiRNA Expression to Uncover Hidden Functions.
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    Chapter 15 Analysis of the Distribution Profiles of Circulating MicroRNAs by Asymmetrical Flow Field Flow Fractionation.
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    Chapter 16 MicroRNA Expression Profiling Using Agilent One-Color Microarray.
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    Chapter 17 A Multiplex Ligation Assay for miRNA Copy Number Profiling.
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    Chapter 18 Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.
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    Chapter 19 Visualization and Analysis of MiRNA-Targets Interactions Networks.
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    Chapter 20 Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.
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    Chapter 21 Customization of Artificial MicroRNA Design.
Attention for Chapter 18: Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.
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Chapter title
Practical Bioinformatics Analysis of MiRNA Data Using Online Tools.
Chapter number 18
Book title
MicroRNA Profiling
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6524-3_18
Pubmed ID
Book ISBNs
978-1-4939-6522-9, 978-1-4939-6524-3
Authors

James A. L. Brown, Emer Bourke

Editors

Sweta Rani

Abstract

The purpose of this chapter is to provide a starting point for the analysis of miRNA array data, using freely available online suites of tools. This chapter does not describe how to perform analysis of primary array data, rather how to use the top differentially regulated miRNA (returned from comparing one miRNA group to another) as the starting point for further practical analysis.Here we describe the methods and tools required to identify targets worthy of additional investigation, using the identified miRNA as a starting point. Importantly, this additional information (pathways targeted, gene expression, mRNA targets, miRNA families) can be used to positively inform any project.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 20%
Researcher 2 13%
Student > Doctoral Student 2 13%
Student > Bachelor 2 13%
Student > Ph. D. Student 1 7%
Other 4 27%
Unknown 1 7%
Readers by discipline Count As %
Medicine and Dentistry 5 33%
Biochemistry, Genetics and Molecular Biology 4 27%
Agricultural and Biological Sciences 4 27%
Arts and Humanities 1 7%
Neuroscience 1 7%
Other 0 0%