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Mendeley readers
Attention Score in Context
| Title |
CORALINA: a universal method for the generation of gRNA libraries for CRISPR-based screening
|
|---|---|
| Published in |
BMC Genomics, November 2016
|
| DOI | 10.1186/s12864-016-3268-z |
| Pubmed ID | |
| Authors |
Anna Köferle, Karolina Worf, Christopher Breunig, Valentin Baumann, Javier Herrero, Maximilian Wiesbeck, Lukas H. Hutter, Magdalena Götz, Christiane Fuchs, Stephan Beck, Stefan H. Stricker |
| Abstract |
The bacterial CRISPR system is fast becoming the most popular genetic and epigenetic engineering tool due to its universal applicability and adaptability. The desire to deploy CRISPR-based methods in a large variety of species and contexts has created an urgent need for the development of easy, time- and cost-effective methods enabling large-scale screening approaches. Here we describe CORALINA (comprehensive gRNA library generation through controlled nuclease activity), a method for the generation of comprehensive gRNA libraries for CRISPR-based screens. CORALINA gRNA libraries can be derived from any source of DNA without the need of complex oligonucleotide synthesis. We show the utility of CORALINA for human and mouse genomic DNA, its reproducibility in covering the most relevant genomic features including regulatory, coding and non-coding sequences and confirm the functionality of CORALINA generated gRNAs. The simplicity and cost-effectiveness make CORALINA suitable for any experimental system. The unprecedented sequence complexities obtainable with CORALINA libraries are a necessary pre-requisite for less biased large scale genomic and epigenomic screens. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 14% |
| Sweden | 1 | 14% |
| Unknown | 5 | 71% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 5 | 71% |
| Scientists | 2 | 29% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 2% |
| Netherlands | 1 | 2% |
| Germany | 1 | 2% |
| Unknown | 57 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 18% |
| Student > Ph. D. Student | 10 | 17% |
| Student > Master | 7 | 12% |
| Student > Doctoral Student | 5 | 8% |
| Student > Bachelor | 4 | 7% |
| Other | 10 | 17% |
| Unknown | 13 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 20 | 33% |
| Agricultural and Biological Sciences | 12 | 20% |
| Chemistry | 3 | 5% |
| Medicine and Dentistry | 2 | 3% |
| Linguistics | 1 | 2% |
| Other | 5 | 8% |
| Unknown | 17 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 13. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 April 2023.
All research outputs
#2,702,539
of 25,698,912 outputs
Outputs from BMC Genomics
#769
of 11,320 outputs
Outputs of similar age
#43,495
of 314,199 outputs
Outputs of similar age from BMC Genomics
#20
of 225 outputs
Altmetric has tracked 25,698,912 research outputs across all sources so far. Compared to these this one has done well and is in the 89th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 11,320 research outputs from this source. They receive a mean Attention Score of 4.8. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 314,199 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 225 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.