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Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state

Overview of attention for article published in Development (09501991), June 2010
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (90th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

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1 blog
3 patents


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201 Mendeley
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Embryonic germ cells from mice and rats exhibit properties consistent with a generic pluripotent ground state
Published in
Development (09501991), June 2010
DOI 10.1242/dev.050427
Pubmed ID

H. G. Leitch, K. Blair, W. Mansfield, H. Ayetey, P. Humphreys, J. Nichols, M. A. Surani, A. Smith


Mouse and rat embryonic stem cells can be sustained in defined medium by dual inhibition (2i) of the mitogen-activated protein kinase (Erk1/2) cascade and of glycogen synthase kinase 3. The inhibitors suppress differentiation and enable self-renewal of pluripotent cells that are ex vivo counterparts of naïve epiblast cells in the mature blastocyst. Pluripotent stem cell lines can also be derived from unipotent primordial germ cells via a poorly understood process of epigenetic reprogramming. These are termed embryonic germ (EG) cells to denote their distinct origin. Here we investigate whether EG cell self-renewal and derivation are supported by 2i. We report that mouse EG cells can be established with high efficiency using 2i in combination with the cytokine leukaemia inhibitory factor (LIF). Furthermore, addition of fibroblast growth factor or stem cell factor is unnecessary using 2i-LIF. The derived EG cells contribute extensively to healthy chimaeric mice, including to the germline. Using the same conditions, we describe the first derivations of EG cells from the rat. Rat EG cells express a similar marker profile to rat and mouse ES cells. They have a diploid karyotype, can be clonally expanded and genetically manipulated, and are competent for multilineage colonisation of chimaeras. These findings lend support to the postulate of a conserved molecular ground state in pluripotent rodent cells. Future research will determine the extent to which this is maintained in other mammals and whether, in some species, primordial germ cells might be a more tractable source than epiblast for the capture of naïve pluripotent stem cells.

Mendeley readers

The data shown below were compiled from readership statistics for 201 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 4 2%
Japan 4 2%
United Kingdom 2 <1%
Singapore 1 <1%
France 1 <1%
Belgium 1 <1%
China 1 <1%
Germany 1 <1%
Portugal 1 <1%
Other 0 0%
Unknown 185 92%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 60 30%
Researcher 55 27%
Student > Master 20 10%
Professor > Associate Professor 12 6%
Professor 10 5%
Other 39 19%
Unknown 5 2%
Readers by discipline Count As %
Agricultural and Biological Sciences 124 62%
Biochemistry, Genetics and Molecular Biology 48 24%
Medicine and Dentistry 14 7%
Neuroscience 2 <1%
Business, Management and Accounting 2 <1%
Other 4 2%
Unknown 7 3%

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2014.
All research outputs
of 11,300,336 outputs
Outputs from Development (09501991)
of 5,149 outputs
Outputs of similar age
of 129,696 outputs
Outputs of similar age from Development (09501991)
of 36 outputs
Altmetric has tracked 11,300,336 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 5,149 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.0. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 129,696 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 36 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.