Title |
Elasmobranch qPCR reference genes: a case study of hypoxia preconditioned epaulette sharks
|
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Published in |
BMC Molecular and Cell Biology, April 2010
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DOI | 10.1186/1471-2199-11-27 |
Pubmed ID | |
Authors |
Kalle T Rytkönen, Gillian MC Renshaw, Kevin J Ashton, Grant Williams-Pritchard, Erica H Leder, Mikko Nikinmaa |
Abstract |
Elasmobranch fishes are an ancient group of vertebrates which have high potential as model species for research into evolutionary physiology and genomics. However, no comparative studies have established suitable reference genes for quantitative PCR (qPCR) in elasmobranchs for any physiological conditions. Oxygen availability has been a major force shaping the physiological evolution of vertebrates, especially fishes. Here we examined the suitability of 9 reference candidates from various functional categories after a single hypoxic insult or after hypoxia preconditioning in epaulette shark (Hemiscyllium ocellatum). |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 3% |
Unknown | 60 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 11 | 18% |
Student > Bachelor | 10 | 16% |
Student > Master | 10 | 16% |
Researcher | 7 | 11% |
Student > Doctoral Student | 4 | 6% |
Other | 8 | 13% |
Unknown | 12 | 19% |
Readers by discipline | Count | As % |
---|---|---|
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Environmental Science | 5 | 8% |
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Medicine and Dentistry | 2 | 3% |
Other | 2 | 3% |
Unknown | 12 | 19% |