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NMR Investigation of Structures of G-protein Coupled Receptor Folding Intermediates

Overview of attention for article published in Journal of Biological Chemistry, December 2016
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Title
NMR Investigation of Structures of G-protein Coupled Receptor Folding Intermediates
Published in
Journal of Biological Chemistry, December 2016
DOI 10.1074/jbc.m116.740985
Pubmed ID
Authors

Martin Poms, Philipp Ansorge, Luis Martinez-Gil, Simon Jurt, Daniel Gottstein, Katrina E. Fracchiolla, Leah S. Cohen, Peter Güntert, Ismael Mingarro, Fred Naider, Oliver Zerbe

Abstract

Folding of G-protein coupled receptors (GPCRs) according to the two-stage model (Popot et al., Biochemistry 29(1990), 4031) is postulated to proceed in 2 steps: Partitioning of the polypeptide into the membrane followed by diffusion until native contacts are formed. Herein we investigate conformational preferences of fragments of the yeast Ste2p receptor using NMR. Constructs comprising the first, the first two and the first three transmembrane (TM) segments, as well as a construct comprising TM1-TM2 covalently linked to TM7 were examined. We observed that the isolated TM1 does not form a stable helix nor does it integrate well into the micelle. TM1 is significantly stabilized upon interaction with TM2, forming a helical hairpin reported previously (Neumoin et al., Biophys. J. 96(2009), 3187), and in this case the protein integrates into the hydrophobic interior of the micelle. TM123 displays a strong tendency to oligomerize, but hydrogen exchange data reveal that the center of TM3 is solvent exposed. In all GPCRs so-far structurally characterized TM7 forms many contacts with TM1 and TM2. In our study TM127 integrates well into the hydrophobic environment, but TM7 does not stably pack against the remaining helices. Topology mapping in microsomal membranes also indicates that TM1 does not integrate in a membrane-spanning fashion, but that TM12, TM123 and TM127 adopt predominantly native-like topologies. The data from our study would be consistent with the retention of individual helices of incompletely synthesized GPCRs in the vicinity of the translocon until the complete receptor is released into the membrane interior.

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Geographical breakdown

Country Count As %
United Kingdom 1 4%
Unknown 25 96%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 31%
Researcher 6 23%
Student > Doctoral Student 3 12%
Student > Bachelor 2 8%
Professor 2 8%
Other 5 19%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 42%
Agricultural and Biological Sciences 7 27%
Chemistry 3 12%
Physics and Astronomy 2 8%
Medicine and Dentistry 1 4%
Other 1 4%
Unknown 1 4%