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Bacterial Cell Wall Homeostasis

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Cover of 'Bacterial Cell Wall Homeostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Bacterial Cell Wall Homeostasis
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    Chapter 2 Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.
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    Chapter 3 Bacterial Cell Wall Homeostasis
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    Chapter 4 Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes.
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    Chapter 5 Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli.
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    Chapter 6 Bacterial Cell Wall Homeostasis
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    Chapter 7 Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
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    Chapter 8 Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products.
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    Chapter 9 Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant.
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    Chapter 10 Bacterial Cell Wall Homeostasis
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    Chapter 11 Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
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    Chapter 12 Bacterial Cell Wall Homeostasis
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    Chapter 13 Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
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    Chapter 14 Analysis of Peptidoglycan Fragment Release.
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    Chapter 15 Bacterial Cell Wall Homeostasis
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    Chapter 16 Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
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    Chapter 17 Biophysical Measurements of Bacterial Cell Shape.
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    Chapter 18 Bacterial Cell Wall Homeostasis
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    Chapter 19 Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.
Attention for Chapter 13: Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
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Chapter title
Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
Chapter number 13
Book title
Bacterial Cell Wall Homeostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3676-2_13
Pubmed ID
Book ISBNs
978-1-4939-3674-8, 978-1-4939-3676-2
Authors

Alexander J. F. Egan, Waldemar Vollmer

Editors

Hee-Jeon Hong

Abstract

Bacterial cell wall peptidoglycan is synthesized from its precursor lipid II by two enzymatic reactions. First, glycosyltransferases polymerize the glycan strands and second, DD-transpeptidases form cross-links between peptides of neighboring strands. Most bacteria possess bifunctional peptidoglycan synthesis enzymes capable of catalyzing both reactions. Here, we describe a continuous fluorescence glycosyltransferase assay using Dansyl-labeled lipid II as substrate. Progression of the reaction is monitored by the reduction in fluorescence over time. The assay is suitable to investigate the effect of protein interaction partners on the glycan strand synthesis activity of peptidoglycan polymerases.

Mendeley readers

The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 13 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 38%
Professor > Associate Professor 3 23%
Researcher 2 15%
Student > Master 1 8%
Student > Doctoral Student 1 8%
Other 1 8%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 46%
Agricultural and Biological Sciences 4 31%
Chemistry 1 8%
Unknown 2 15%