| Chapter title |
Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
|
|---|---|
| Chapter number | 13 |
| Book title |
Bacterial Cell Wall Homeostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3676-2_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3674-8, 978-1-4939-3676-2
|
| Authors |
Alexander J. F. Egan, Waldemar Vollmer |
| Editors |
Hee-Jeon Hong |
| Abstract |
Bacterial cell wall peptidoglycan is synthesized from its precursor lipid II by two enzymatic reactions. First, glycosyltransferases polymerize the glycan strands and second, DD-transpeptidases form cross-links between peptides of neighboring strands. Most bacteria possess bifunctional peptidoglycan synthesis enzymes capable of catalyzing both reactions. Here, we describe a continuous fluorescence glycosyltransferase assay using Dansyl-labeled lipid II as substrate. Progression of the reaction is monitored by the reduction in fluorescence over time. The assay is suitable to investigate the effect of protein interaction partners on the glycan strand synthesis activity of peptidoglycan polymerases. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 44% |
| Professor > Associate Professor | 3 | 17% |
| Researcher | 2 | 11% |
| Student > Master | 2 | 11% |
| Student > Bachelor | 1 | 6% |
| Other | 2 | 11% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 9 | 50% |
| Agricultural and Biological Sciences | 5 | 28% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
| Chemistry | 1 | 6% |
| Unknown | 2 | 11% |