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Bacterial Cell Wall Homeostasis

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Cover of 'Bacterial Cell Wall Homeostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Bacterial Cell Wall Homeostasis
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    Chapter 2 Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.
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    Chapter 3 Bacterial Cell Wall Homeostasis
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    Chapter 4 Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes.
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    Chapter 5 Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli.
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    Chapter 6 Bacterial Cell Wall Homeostasis
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    Chapter 7 Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
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    Chapter 8 Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products.
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    Chapter 9 Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant.
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    Chapter 10 Bacterial Cell Wall Homeostasis
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    Chapter 11 Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
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    Chapter 12 Bacterial Cell Wall Homeostasis
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    Chapter 13 Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
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    Chapter 14 Analysis of Peptidoglycan Fragment Release.
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    Chapter 15 Bacterial Cell Wall Homeostasis
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    Chapter 16 Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
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    Chapter 17 Biophysical Measurements of Bacterial Cell Shape.
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    Chapter 18 Bacterial Cell Wall Homeostasis
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    Chapter 19 Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.
Attention for Chapter 14: Analysis of Peptidoglycan Fragment Release.
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Chapter title
Analysis of Peptidoglycan Fragment Release.
Chapter number 14
Book title
Bacterial Cell Wall Homeostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3676-2_14
Pubmed ID
Book ISBNs
978-1-4939-3674-8, 978-1-4939-3676-2
Authors

Ryan E. Schaub, Jonathan D. Lenz, Joseph P. Dillard

Editors

Hee-Jeon Hong

Abstract

Most bacteria break down a significant portion of their cell wall peptidoglycan during each round of growth and cell division. This process generates peptidoglycan fragments of various sizes that can either be imported back into the cytoplasm for recycling or released from the cell. Released fragments have been shown to act as microbe-associated molecular patterns for the initiation of immune responses, as triggers for the initiation of mutualistic host-microbe relationships, and as signals for cell-cell communication in bacteria. Characterizing these released peptidoglycan fragments can, therefore, be considered an important step in understanding how microbes communicate with other organisms in their environments. In this chapter, we describe methods for labeling cell wall peptidoglycan, calculating the rate at which peptidoglycan is turned over, and collecting released peptidoglycan to determine the abundance and species of released fragments. Methods are described for both the separation of peptidoglycan fragments by size-exclusion chromatography and further detailed analysis by HPLC.

Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 2 20%
Student > Ph. D. Student 2 20%
Professor 1 10%
Student > Doctoral Student 1 10%
Student > Bachelor 1 10%
Other 1 10%
Unknown 2 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 50%
Pharmacology, Toxicology and Pharmaceutical Science 1 10%
Agricultural and Biological Sciences 1 10%
Chemistry 1 10%
Unknown 2 20%