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Bacterial Cell Wall Homeostasis

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Cover of 'Bacterial Cell Wall Homeostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Bacterial Cell Wall Homeostasis
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    Chapter 2 Ultra-Sensitive, High-Resolution Liquid Chromatography Methods for the High-Throughput Quantitative Analysis of Bacterial Cell Wall Chemistry and Structure.
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    Chapter 3 Bacterial Cell Wall Homeostasis
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    Chapter 4 Cell Shaving and False-Positive Control Strategies Coupled to Novel Statistical Tools to Profile Gram-Positive Bacterial Surface Proteomes.
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    Chapter 5 Differential Proteomics Based on Multidimensional Protein Identification Technology to Understand the Biogenesis of Outer Membrane of Escherichia coli.
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    Chapter 6 Bacterial Cell Wall Homeostasis
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    Chapter 7 Zymographic Techniques for the Analysis of Bacterial Cell Wall in Bacillus.
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    Chapter 8 Liquid Chromatography-Tandem Mass Spectrometry to Define Sortase Cleavage Products.
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    Chapter 9 Genetics and Cell Morphology Analyses of the Actinomyces oris srtA Mutant.
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    Chapter 10 Bacterial Cell Wall Homeostasis
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    Chapter 11 Luciferase Reporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.
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    Chapter 12 Bacterial Cell Wall Homeostasis
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    Chapter 13 Continuous Fluorescence Assay for Peptidoglycan Glycosyltransferases.
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    Chapter 14 Analysis of Peptidoglycan Fragment Release.
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    Chapter 15 Bacterial Cell Wall Homeostasis
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    Chapter 16 Analysis of Bacterial Cell Surface Chemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.
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    Chapter 17 Biophysical Measurements of Bacterial Cell Shape.
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    Chapter 18 Bacterial Cell Wall Homeostasis
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    Chapter 19 Structural Comparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.
Attention for Chapter 17: Biophysical Measurements of Bacterial Cell Shape.
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Chapter title
Biophysical Measurements of Bacterial Cell Shape.
Chapter number 17
Book title
Bacterial Cell Wall Homeostasis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3676-2_17
Pubmed ID
Book ISBNs
978-1-4939-3674-8, 978-1-4939-3676-2
Authors

Jeffrey P. Nguyen, Benjamin P. Bratton, Joshua W. Shaevitz

Editors

Hee-Jeon Hong

Abstract

A bacteria's shape plays a large role in determining its mechanism of motility, energy requirements, and ability to avoid predation. Although it is a major factor in cell fitness, little is known about how cell shape is determined or maintained. These problems are made worse by a lack of accurate methods to measure cell shape in vivo, as current methods do not account for blurring artifacts introduced by the microscope. Here, we introduce a method using 2D active surfaces and forward convolution with a measured point spread function to measure the 3D shape of different strains of E. coli from fluorescent images. Using this technique, we are also able to measure the distribution of fluorescent molecules, such as polymers, on the cell surface. This quantification of the surface geometry and fluorescence distribution allow for a more precise measure of 3D cell shape and is a useful tool for measuring protein localization and the mechanisms of bacterial shape control.

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 29%
Student > Doctoral Student 2 14%
Professor 2 14%
Student > Ph. D. Student 2 14%
Other 1 7%
Other 3 21%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 5 36%
Physics and Astronomy 4 29%
Immunology and Microbiology 2 14%
Agricultural and Biological Sciences 2 14%
Engineering 1 7%
Other 0 0%