| Chapter title |
Bacterial Cell Wall Homeostasis
|
|---|---|
| Chapter number | 6 |
| Book title |
Bacterial Cell Wall Homeostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3676-2_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3674-8, 978-1-4939-3676-2
|
| Authors |
Reyes-Cortés, Ruth, Arguijo-Hernández, Emma S, Carballo-Ontiveros, Marco A, Martínez-Peñafiel, Eva, Kameyama, Luis, Ruth Reyes-Cortés, Emma S. Arguijo-Hernández, Marco A. Carballo-Ontiveros, Eva Martínez-Peñafiel, Luis Kameyama |
| Editors |
Hee-Jeon Hong |
| Abstract |
In order to identify host components involved in the infective process of bacteriophages, we developed a wide-range strategy to obtain cell envelope mutants, using Escherichia coli W3110 and its specific phage mEp213. The strategy consisted in four steps: (1) random mutagenesis using transposon miniTn10Km(r); (2) selection of phage-resistant mutants by replica-plating; (3) electroporation of the phage-resistant mutants with mEp213 genome, followed by selection of those allowing phage development; and (4) sequencing of the transposon-disrupted genes. This strategy allowed us to distinguish the host factors related to phage development or multiplication within the cell, from those involved in phage infection at the level of the cell envelope. |
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