| Chapter title |
Bacterial Cell Wall Homeostasis
|
|---|---|
| Chapter number | 15 |
| Book title |
Bacterial Cell Wall Homeostasis
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3676-2_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3674-8, 978-1-4939-3676-2
|
| Authors |
Covas, Gonçalo, Vaz, Filipa, Henriques, Gabriela, Pinho, Mariana G, Filipe, Sérgio R, Gonçalo Covas, Filipa Vaz, Gabriela Henriques, Mariana G. Pinho, Sérgio R. Filipe |
| Editors |
Hee-Jeon Hong |
| Abstract |
Most bacterial cells are surrounded by a surface composed mainly of peptidoglycan (PGN), a glycopolymer responsible for ensuring the bacterial shape and a telltale molecule that betrays the presence of bacteria to the host immune system. In Staphylococcus aureus, as in most gram-positive bacteria, peptidoglycan is concealed by covalently linked molecules of wall teichoic acids (WTA)-phosphate rich molecules made of glycerol and ribitol phosphates which may be tailored by different amino acids and sugars.In order to analyze and compare the composition of WTA produced by different S. aureus strains, we describe methods to: (1) quantify the total amount of WTA present at the bacterial cell surface, through the determination of the inorganic phosphate present in phosphodiester linkages of WTA; (2) identify which sugar constituents are present in the assembled WTA molecules, by detecting the monosaccharides, released by acid hydrolysis, through an high-performance anion exchange chromatography analysis coupled with pulsed amperometric detection (HPAEC-PAD) and (3) compare the polymerization degree of WTA found at the cell surface of different S. aureus strains, through their different migration in a polyacrylamide gel electrophoresis (PAGE). |
Mendeley readers
Geographical breakdown
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|---|---|---|
| Portugal | 1 | 3% |
| Unknown | 30 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 16% |
| Student > Postgraduate | 5 | 16% |
| Student > Ph. D. Student | 4 | 13% |
| Student > Doctoral Student | 3 | 10% |
| Student > Bachelor | 3 | 10% |
| Other | 5 | 16% |
| Unknown | 6 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Agricultural and Biological Sciences | 4 | 13% |
| Medicine and Dentistry | 2 | 6% |
| Chemical Engineering | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 7 | 23% |