| Chapter title |
Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
|
|---|---|
| Chapter number | 6 |
| Book title |
Antibiotics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6634-9_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6632-5, 978-1-4939-6634-9
|
| Authors |
Alexander Zipperer, Dorothee Kretschmer |
| Editors |
Peter Sass |
| Abstract |
The development of safe antimicrobial agents is important for the effective treatment of pathogens. From a multitude of discovered inhibitory compounds only few antimicrobial agents are able to enter the market. Many antimicrobials are, on the one hand, quite effective in killing pathogens but, on the other hand, cytotoxic to eukaryotic cells. Cell health can be monitored by various methods. Plasma membrane integrity, DNA synthesis, enzyme activity, and reducing conditions within the cell are known indicators of cell viability and cell death. For a comprehensive overview, methods to analyze cytotoxic and hemolytic effects, e.g., lactate dehydrogenase release, cell proliferation analysis, cell viability analysis, and hemolysis assay of antimicrobial compounds on human cells, are described in this chapter. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Student > Doctoral Student | 2 | 29% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Master | 1 | 14% |
| Unknown | 1 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 29% |
| Medicine and Dentistry | 2 | 29% |
| Agricultural and Biological Sciences | 1 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 14% |
| Unknown | 1 | 14% |