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Antibiotics

Overview of attention for book
Cover of 'Antibiotics'

Table of Contents

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    Book Overview
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    Chapter 1 Antibiotics: Precious Goods in Changing Times
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    Chapter 2 Mining Bacterial Genomes for Secondary Metabolite Gene Clusters
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    Chapter 3 Production of Antimicrobial Compounds by Fermentation
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    Chapter 4 Structure Elucidation of Antibiotics by NMR Spectroscopy
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    Chapter 5 Computer-Aided Drug Design Methods
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    Chapter 6 Cytotoxicity Assays as Predictors of the Safety and Efficacy of Antimicrobial Agents
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    Chapter 7 Application of a Bacillus subtilis Whole-Cell Biosensor (PliaI-lux) for the Identification of Cell Wall Active Antibacterial Compounds
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    Chapter 8 Determination of Bacterial Membrane Impairment by Antimicrobial Agents
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    Chapter 9 Mass-Sensitive Biosensor Systems to Determine the Membrane Interaction of Analytes
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    Chapter 10 Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy
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    Chapter 11 In Vitro Assays to Identify Antibiotics Targeting DNA Metabolism
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    Chapter 12 Fluorescence-Based Real-Time Activity Assays to Identify RNase P Inhibitors
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    Chapter 13 Reporter Gene-Based Screening for TPP Riboswitch Activators
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    Chapter 14 Cell-Based Fluorescent Screen to Identify Inhibitors of Bacterial Translation Initiation
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    Chapter 15 Bacterial Histidine Kinases: Overexpression, Purification, and Inhibitor Screen
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    Chapter 16 Expression Profiling of Antibiotic-Resistant Bacteria Obtained by Laboratory Evolution
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    Chapter 17 Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research
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    Chapter 18 Label-Free Quantitation of Ribosomal Proteins from Bacillus subtilis for Antibiotic Research
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    Chapter 19 Functional Metagenomics to Study Antibiotic Resistance
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    Chapter 20 Epidemiological Surveillance and Typing Methods to Track Antibiotic Resistant Strains Using High Throughput Sequencing
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    Chapter 21 Erratum
Attention for Chapter 10: Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy
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Citations

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Chapter title
Measurement of Cell Membrane Fluidity by Laurdan GP: Fluorescence Spectroscopy and Microscopy
Chapter number 10
Book title
Antibiotics
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-6634-9_10
Pubmed ID
27873252
Book ISBNs
978-1-4939-6632-5, 978-1-4939-6634-9
Authors

Kathi Scheinpflug, Oxana Krylova, Henrik Strahl

Editors

Peter Sass

Abstract

Membrane fluidity is a critical parameter of cellular membranes which cells continuously strive to maintain within a viable range. An interference with the correct membrane fluidity state can strongly inhibit cell function. Triggered changes in membrane fluidity have been postulated to contribute to the mechanism of action of membrane targeting antimicrobials, but the corresponding analyses have been hampered by the absence of readily available analytical tools. Here, we provide detailed protocols that allow straightforward measurement of antibiotic compound-triggered changes in membrane fluidity both in vivo and in vitro.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 69 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 69 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 16%
Researcher 10 14%
Student > Master 10 14%
Student > Bachelor 8 12%
Student > Doctoral Student 5 7%
Other 11 16%
Unknown 14 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 18 26%
Agricultural and Biological Sciences 15 22%
Immunology and Microbiology 4 6%
Chemistry 4 6%
Pharmacology, Toxicology and Pharmaceutical Science 3 4%
Other 9 13%
Unknown 16 23%

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