You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Chapter title |
Sample Preparation for Mass-Spectrometry Based Absolute Protein Quantification in Antibiotic Stress Research
|
|---|---|
| Chapter number | 17 |
| Book title |
Antibiotics
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6634-9_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6632-5, 978-1-4939-6634-9
|
| Authors |
Florian Bonn, Sandra Maass, Dörte Becher |
| Editors |
Peter Sass |
| Abstract |
Absolute protein quantification is an essential tool for system biology approaches and elucidation of stoichiometry of multi-protein complexes. In this chapter, a universal protocol for gel free absolute protein quantification in bacterial systems is described, which can be used for sample preparation prior to miscellaneous mass-spectrometry-based quantification workflows like AQUA, Hi3, and emPAI. In addition, a focus has been set to the specific challenges in antibiotic stress research. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Other | 1 | 17% |
| Student > Bachelor | 1 | 17% |
| Student > Master | 1 | 17% |
| Researcher | 1 | 17% |
| Professor > Associate Professor | 1 | 17% |
| Other | 0 | 0% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Computer Science | 2 | 33% |
| Biochemistry, Genetics and Molecular Biology | 1 | 17% |
| Agricultural and Biological Sciences | 1 | 17% |
| Engineering | 1 | 17% |
| Unknown | 1 | 17% |