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Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57’) storage roots and their synthesized peptides

Overview of attention for article published in Botanical Studies, August 2014
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Title
Antioxidant activities of two metallothionein-like proteins from sweet potato (Ipomoea batatas [L.] Lam. `Tainong 57’) storage roots and their synthesized peptides
Published in
Botanical Studies, August 2014
DOI 10.1186/s40529-014-0064-4
Pubmed ID
Authors

Shyh-Shyun Huang, Jeng-Shyan Deng, Hsien-Jung Chen, Yaw-Huei Lin, Guan-Jhong Huang

Abstract

Metallothionein (MT) characterized by their low molecular weight and high cysteine content. Two recombinant proteins of MT-I and MT-II overproduced in E. coli (M15) was purified by Ni(2+)-chelated affinity chromatography. The molecular mass of MT-I and MT-II are ca. 6,600 and 8,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total antioxidant status, DPPH radical scavenging activity, reducing power method, Fe(2+)-chelating ability, ferric thiocyanate (FTC) method, and protecting calf thymus DNA against hydroxyl radical-induced damage were studied. The MT-I and MT-II proteins with a concentration of 100 μg/mL exhibited the highest activity (expressed respectively as 61.72 ± 0.13 and 74.28 ± 1.15 μM Trolox equivalent antioxidative capacity, TEAC) in total antioxidant status test. Like total antioxidant status, DPPH radical scavenging activity, reducing power, Fe(2+)-chelating ability, FTC activity, and protecting calf thymus DNA against hydroxyl radical-induced damage all showed that MT-1 and MT-II proteins have antioxidant activities. In this study, we also found that antioxidant activities of MT-I and MT-II increased from 17% and 16% (0 h) to about 26% and 28% (24 h) after 24 h hydrolysis by trypsin. Smaller peptides increased the antioxidant activities. Four and three peptides, respectively, from MT-I and MT-II protein sequences for testing antioxidative activity were synthesized according to tryptic hydrolysis simulation. The obtained MSSGCK, CGSDCK, LTLEGSSEK, ATEGGHACK, CGNGCGGCK, and CDPCNCK showed IC50 values of 309.87, 1423.37, 3925.54, 561.32, 300.76, and 610.12 μM, respectively, when scavenging activity of DPPH radicals (%) was measured. These findings mean that a cysteine residue is most important in antiradical activities. It was suggested that MT-I and MT-II might contribute their antioxidant activities against hydroxyl and peroxyl radicals.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 29 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Algeria 1 3%
Unknown 28 97%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 7 24%
Student > Master 5 17%
Student > Bachelor 4 14%
Professor 2 7%
Student > Doctoral Student 1 3%
Other 5 17%
Unknown 5 17%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 21%
Biochemistry, Genetics and Molecular Biology 5 17%
Chemistry 4 14%
Pharmacology, Toxicology and Pharmaceutical Science 2 7%
Medicine and Dentistry 2 7%
Other 2 7%
Unknown 8 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 November 2016.
All research outputs
#15,982,037
of 25,371,288 outputs
Outputs from Botanical Studies
#75
of 188 outputs
Outputs of similar age
#129,887
of 243,217 outputs
Outputs of similar age from Botanical Studies
#1
of 3 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one is in the 36th percentile – i.e., 36% of other outputs scored the same or lower than it.
So far Altmetric has tracked 188 research outputs from this source. They receive a mean Attention Score of 4.5. This one has gotten more attention than average, scoring higher than 60% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 243,217 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 46th percentile – i.e., 46% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them