Chapter title |
Analysis of EBV Transcription Using High-Throughput RNA Sequencing.
|
---|---|
Chapter number | 7 |
Book title |
Epstein Barr Virus
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6655-4_7 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6653-0, 978-1-4939-6655-4
|
Authors |
Tina O’Grady, Melody Baddoo, Erik K. Flemington, O'Grady, Tina, Baddoo, Melody, Flemington, Erik K. |
Editors |
Janos Minarovits, Hans Helmut Niller |
Abstract |
High-throughput sequencing of RNA is used to analyze the transcriptomes of viruses and cells, providing information about transcript structure and abundance. A wide array of programs and pipelines has been created to manage and interpret the abundance of data generated from high-throughput RNA sequencing experiments. This protocol details the use of free and open-source programs to align RNA-Seq reads to a reference genome, visualize read coverage and splice junctions, estimate transcript abundance, and evaluate differential expression of transcripts in different conditions. Particular concerns related to EBV and viral transcriptomics are addressed and access to EBV reference files is provided. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
United States | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 3 | 27% |
Student > Doctoral Student | 2 | 18% |
Researcher | 2 | 18% |
Student > Master | 2 | 18% |
Student > Ph. D. Student | 1 | 9% |
Other | 0 | 0% |
Unknown | 1 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 27% |
Medicine and Dentistry | 3 | 27% |
Agricultural and Biological Sciences | 2 | 18% |
Environmental Science | 1 | 9% |
Unknown | 2 | 18% |