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Mendeley readers
| Chapter title |
Analysis of Viral Epigenotypes Using Bisulfite Sequencing: A Detailed Protocol for the Crucial Bisulfite Modification and PCR Amplification Steps.
|
|---|---|
| Chapter number | 15 |
| Book title |
Epstein Barr Virus
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6655-4_15 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6653-0, 978-1-4939-6655-4
|
| Authors |
Daniel Salamon |
| Editors |
Janos Minarovits, Hans Helmut Niller |
| Abstract |
Characterization of viral DNA methylation patterns is essential to understand its function in viral pathogenesis. Bisulfite modification, followed by polymerase chain reaction (PCR) and sequencing is the most effective method for the high resolution methylation mapping of viral genomes. Since the bisulfite modification and PCR steps are the most critical ones, an optimized protocol for these two steps is presented, with special attention to potential pitfalls. |
Mendeley readers
The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Indonesia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 100% |
| Readers by discipline | Count | As % |
|---|---|---|
| Nursing and Health Professions | 1 | 50% |
| Medicine and Dentistry | 1 | 50% |