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Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase

Overview of attention for article published in BMC Biotechnology, January 2012
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1 tweeter

Citations

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Title
Cloning, sequence analysis, expression of Cyathus bulleri laccase in Pichia pastoris and characterization of recombinant laccase
Published in
BMC Biotechnology, January 2012
DOI 10.1186/1472-6750-12-75
Pubmed ID
Authors

Neha Garg, Nora Bieler, Tenzin Kenzom, Meenu Chhabra, Marion Ansorge-Schumacher, Saroj Mishra

Abstract

Laccases are blue multi-copper oxidases and catalyze the oxidation of phenolic and non-phenolic compounds. There is considerable interest in using these enzymes for dye degradation as well as for synthesis of aromatic compounds. Laccases are produced at relatively low levels and, sometimes, as isozymes in the native fungi. The investigation of properties of individual enzymes therefore becomes difficult. The goal of this study was to over-produce a previously reported laccase from Cyathus bulleri using the well-established expression system of Pichia pastoris and examine and compare the properties of the recombinant enzyme with that of the native laccase.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
South Africa 1 1%
Unknown 80 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 21 26%
Student > Bachelor 15 19%
Student > Master 13 16%
Researcher 11 14%
Student > Doctoral Student 7 9%
Other 10 12%
Unknown 4 5%
Readers by discipline Count As %
Agricultural and Biological Sciences 42 52%
Biochemistry, Genetics and Molecular Biology 18 22%
Environmental Science 4 5%
Engineering 3 4%
Business, Management and Accounting 2 2%
Other 4 5%
Unknown 8 10%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 April 2013.
All research outputs
#9,906,227
of 12,373,386 outputs
Outputs from BMC Biotechnology
#557
of 695 outputs
Outputs of similar age
#99,519
of 143,509 outputs
Outputs of similar age from BMC Biotechnology
#2
of 3 outputs
Altmetric has tracked 12,373,386 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 695 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.1. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 143,509 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 16th percentile – i.e., 16% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3 others from the same source and published within six weeks on either side of this one.