| Chapter title |
An Assay for Measuring Histone Variant Exchange within Nucleosomes In Vitro.
|
|---|---|
| Chapter number | 2 |
| Book title |
Histones
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6630-1_2 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6628-8, 978-1-4939-6630-1
|
| Authors |
Liette Laflamme, Benoit Guillemette, Luc Gaudreau |
| Editors |
Benoit Guillemette, Luc R. Gaudreau |
| Abstract |
The incorporation of histone variants into specific chromatin regions is a mechanism by which cells can regulate many important biological processes. One such example is H2A.Z, a highly conserved variant of H2A that is incorporated in genomic regulatory regions and contributes to control gene expression. H2A.Z variant exchange involves the removal of H2A-H2B dimers from a preassembled nucleosome and their replacement with H2A.Z-H2B dimers. A specific family of chromatin remodeling complexes, homologous to the yeast Swr1 complex, have been shown to be capable of this histone exchange activity both in vivo and in vitro. Here, we describe an assay to measure the histone H2A.Z exchange activity of recombinant human p400 on immobilized mononucleosomes in vitro. The assay can be adapted to other histone exchange complexes/catalytic subunits purified from any species. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 6 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 33% |
| Professor > Associate Professor | 1 | 17% |
| Researcher | 1 | 17% |
| Student > Master | 1 | 17% |
| Unknown | 1 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 2 | 33% |
| Agricultural and Biological Sciences | 2 | 33% |
| Unknown | 2 | 33% |